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DC Field | Value | Language |
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dc.contributor.author | Wany, Aakanksha | - |
dc.contributor.author | Pathak, Pradeep Kumar | - |
dc.contributor.author | Gupta, Kapuganti Jagadis | - |
dc.date.accessioned | 2019-10-15T07:54:26Z | - |
dc.date.available | 2019-10-15T07:54:26Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Methods in Molecular Biology, 2057: 15-26 | en_US |
dc.identifier.isbn | 978-1-4939-9790-9 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1003 | - |
dc.description | Accepted date: 09 October 2019 | en_US |
dc.description.abstract | Nitrogen (N) is one of the most important nutrients which exist in both inorganic and organic forms. Plants assimilate inorganic form of N [nitrate (NO3−), nitrite (NO2−) or ammonium (NH4+)] and incorporate into amino acids. The metabolism of N involves a series of events such as sensing, uptake, and assimilation. The initial stage is sensing, triggered by nitrate or ammonium signals initiating signal transduction processes in N metabolism. The assimilation pathway initiates with NO3−/NH4+ transport to roots via specific high and low affinity (HATs and LATs) nitrate transporters or directly via ammonium transporters (AMTs). In cytosol the NO3− is reduced to NO2− by cytosolic nitrate reductase (NR) and the produced NO2− is further reduced to NH4+ by nitrite reductase (NiR) in plastids. NR has capability to reduce NO2− to nitric oxide (NO) under specific conditions such as hypoxia, low pH, and pathogen infection. The produced NO acts as a signal for wide range of processes such as plant growth development and stress. Here, we provide methods to measure NR activity, NO2− levels, and NO production in plant tissues. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature Publishing AG | en_US |
dc.subject | DAF-FM | en_US |
dc.subject | Nitrate | en_US |
dc.subject | Nitrate reductase | en_US |
dc.subject | Nitrite | en_US |
dc.subject | Nitric oxide | en_US |
dc.title | Methods for measuring nitrate reductase, nitrite levels, and nitric oxide from plant tissues | en_US |
dc.type | Book chapter | en_US |
dc.identifier.officialurl | https://link.springer.com/protocol/10.1007/978-1-4939-9790-9_2 | en_US |
dc.identifier.doi | https://doi.org/10.1007/978-1-4939-9790-9_2 | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Gupta KJ_2021_12.pdf Restricted Access | 397.61 kB | Adobe PDF | View/Open Request a copy |
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