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DC Field | Value | Language |
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dc.contributor.author | Vivek, A.T. | - |
dc.contributor.author | Zahra, Shafaque | - |
dc.contributor.author | Kumar, Shailesh | - |
dc.date.accessioned | 2019-11-04T09:46:10Z | - |
dc.date.available | 2019-11-04T09:46:10Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Methods, 183: 30-37 | en_US |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1011 | - |
dc.description | Accepted date: 22 October 2019 | en_US |
dc.description.abstract | Plants have evolved many defense strategies for combating viral infections. One major surveillance strategy adopted by them is manipulating viral sequences to generate distinct small RNA products via Dicer-like enzymes (DCL), and thereby restricting virus multiplication through the RNA interference (RNAi) mechanism. The power of high-throughput sequencing technologies, with diverse computational tools to handle small RNA sequencing (sRNA-Seq) data, bestows unprecedented opportunities to answer fundamental questions in plant virology. Here, we present some basic concepts of virus-derived, small interfering RNA (vsiRNA) biogenesis in plants, optimization strategies, caveats, and best practices for efficient discovery and diagnosis of known as well as novel plant viruses/viroids using deep sequencing of small RNA (sRNA) pools. | en_US |
dc.description.sponsorship | A.T. Vivek and Shafaque Zahra acknowledge DBT and CSIR, respectively, for providing fellowship support. The authors have used the BioRender tool for image creation. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.subject | Bioinformatics | en_US |
dc.subject | Deep sequencing | en_US |
dc.subject | vsiRNAs | en_US |
dc.subject | plant viruses/viroids | en_US |
dc.subject | viral diagnostics | en_US |
dc.title | From current knowledge to best practice: A primer on Viral diagnostics using deep sequencing of virus-derived small interfering RNAs (vsiRNAs) in infected plants | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://www.sciencedirect.com/science/article/pii/S1046202319301744?via%3Dihub | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.ymeth.2019.10.009 | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Kumar Shai_2019_5.pdf Restricted Access | 1.08 MB | Adobe PDF | View/Open Request a copy |
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