Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1036
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dc.contributor.authorGupta, Aarti-
dc.contributor.authorPatil, Mahesh-
dc.contributor.authorQamar, Aarzoo-
dc.contributor.authorSenthil-Kumar, Muthappa-
dc.date.accessioned2020-01-29T08:59:59Z-
dc.date.available2020-01-29T08:59:59Z-
dc.date.issued2020-
dc.identifier.citationEnvironmental and Experimental Botany, 172: 103998en_US
dc.identifier.issn0098-8472-
dc.identifier.otherhttps://doi.org/10.1016/j.envexpbot.2020.103998-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0098847220300241-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/1036-
dc.descriptionAccepted date: 22 January 2020en_US
dc.description.abstractPlants under combined stresses exhibit a prominent shift in molecular responses compared with plants exposed to the same stresses independently. Profiling responses to individual and combined stressors at the gene expression level have identified several genes with intersecting responses to these stressors. However, the upstream regulators at the intersection of plant responses to individual and combined stresses are not known. Here, using the transcriptome of Arabidopsis thaliana under individual and combined drought and Pseudomonas syringae infection, we identified several genes whose expression overlaps between individual and combined stresses. To study the key regulator of such an overlapping gene, we predicted that the expression of 1-Pyrroline-5-carboxylate synthase 1 (AtP5CS1) is regulated by ath-miR164c at post-transcriptional level. Our results from the stem-loop RT-PCR based expression analysis revealed significant downregulation of ath-miR164c in response to P. syringae infection under both well-irrigated (pathogen only) and drought stress (combined stress) conditions. Furthermore, an Arabidopsis loss-of-function mutant of the miRNA ath-miR164c exhibited resistance to pathogen infection under combined stress, unlike the wild-type plants, implicating the role of ath-miR164c in regulating plant immunity. AtP5CS1 gene expression and proline accumulation were enhanced in the ath-miR164c mutant plants relative to the wild-type plants, demonstrating that ath-miR164c regulates AtP5CS1 of the proline biosynthesis pathway, which was also validated by 5’RLM-RACE results. This miRNA-mediated modulation of AtP5CS1 gene expression under combined stress fills crucial gaps in identifying the key convergent players in the current understanding of plant stress responses.en_US
dc.description.sponsorshipProjects at MS-K lab are supported by DBT-Ramalingaswami re-entry fellowship grant (BT/RLF/re-entry/23/2012). AG (N-PDF/2015/000116) and MP (PDF/2016/000528) acknowledge the SERB National Post-Doctoral Fellowship. We also thank Mr. Sundar and Mr Rahim for extending labour at the laboratory and Mr. Ashok Kumar for technical help at central instrumentation facility. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. NIPGR Plant Growth Facility is also duly acknowledged. We thank Dr Muthamilarasan Mehanathan and Dr William Truman for critical comments on the manuscript. Also, we thank Dr Priya, Mr Vadivel, and Miss Aanchal for internally reviewing the manuscript.en_US
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.subjectDroughten_US
dc.subjectPseudomonas syringaeen_US
dc.subjectPhysiological stressen_US
dc.subjectmicroRNAen_US
dc.subjectProlineen_US
dc.titleath-miR164c influences plant responses to the combined stress of drought and bacterial infection by regulating proline metabolismen_US
dc.typeArticleen_US
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