Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1089
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dc.contributor.authorSharma, Namisha-
dc.contributor.authorPrasad, Manoj-
dc.date.accessioned2020-09-01T10:43:39Z-
dc.date.available2020-09-01T10:43:39Z-
dc.date.issued2020-
dc.identifier.citationPlant Cell Reports, 39: 1565–1579en_US
dc.identifier.issn1432-203X-
dc.identifier.otherhttps://doi.org/10.1007/s00299-020-02584-2-
dc.identifier.urihttps://link.springer.com/article/10.1007/s00299-020-02584-2-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/1089-
dc.descriptionAccepted date: 20 August 2020en_US
dc.description.abstractKey message: Expression of artificial microRNA targeting ATP binding domain of AC1 in transgenic tomato confers resistance to Tomato leaf curl disease without impacting the yield of tomato. Abstract: Tomato curl leaf disease caused by Tomato leaf curl virus (ToLCV) is a key constraint to tomato cultivation worldwide. Engineering transgenic plants expressing artificial microRNAs (amiRNAs) against the AC1 gene of Tomato leaf curl New Delhi virus (ToLCNDV), which is important for virus replication and pathogenicity, would consequently confer virus resistance and reduce crop loss in the economically important crops. This study relates to an amiRNA developed on the sequence of Arabidopsis miRNA319a, targeting the ATP/GTP binding domain of AC1 gene of ToLCNDV. The AC1-amiR was found to regulate the abundance of AC1, providing an excellent strategy in providing defense against ToLCNDV. Transgenic lines over-expressing AC1-amiR, when challenged with ToLCNDV, showed reduced disease symptoms and high percentage resistance ranging between ∼ 40 and 80%. The yield of transgenic plants was significantly higher upon ToLCNDV infection as compared to the non-transgenic plants. Although the natural resistance resources against ToLCNDV are not available, this work streamlines a novel amiRNA-based mechanism that may have the potential to develop viral resistance strategies in tomato, apart from its normal symptom development properties as it is targeting the conserved region against which higher accumulation of small interfering RNAs (siRNA) occurred in a naturally tolerant tomato cultivar.en_US
dc.description.sponsorshipThe authors’ work in the area of plant–virus interaction is supported by the JC Bose Fellowship (JCB/2018/00000l) from Science and Engineering Research Board (SERB), Govt. of India, India and core grant of National Institute of Plant Genome Research (NIPGR), New Delhi. The authors thank Dr. Muthamilarasan Mehanathan, School of Life Sciences, University of Hyderabad, India, for critically reading the manuscript. The authors are also thankful to DBTeLibrary Consortium (DeLCON) for providing access to e-resources.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Nature Publishing AGen_US
dc.subjectArtifcial microRNAen_US
dc.subjectTomato leaf curl diseaseen_US
dc.subjectTomato leaf curl New Delhi virusen_US
dc.subjectVirus resistanceen_US
dc.subjectReplication initiator proteinen_US
dc.titleSilencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomatoen_US
dc.typeArticleen_US
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