Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/1154
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gautam, Vibhav | - |
dc.contributor.author | Chatterjee, Sourav | - |
dc.contributor.author | Sarkar, Ananda K. | - |
dc.date.accessioned | 2021-01-27T05:36:30Z | - |
dc.date.available | 2021-01-27T05:36:30Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Methods in Molecular Biology, 2238: 275-283 | en_US |
dc.identifier.isbn | 978-1-0716-1067-1 | - |
dc.identifier.other | https://doi.org/10.1007/978-1-0716-1068-8_18 | - |
dc.identifier.uri | https://link.springer.com/protocol/10.1007%2F978-1-0716-1068-8_18 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1154 | - |
dc.description | Accepted date: 21 January 2021 | en_US |
dc.description.abstract | The success of single cell type-specific gene expression or functional study largely depends on the efficient isolation of high-quality RNA from them. Laser capture microdissection (LCM) is an efficient technique that allows accessing and dissecting out a specific individual cell or cell type from a microscopic heterogeneous tissue in a minimally disruptive way. Here, we describe an efficient and inexpensive LCM-based method for the extraction of RNAs with high yield and integrity from laser-microdissected mesophyll and bundle sheath cells of rice leaf. The integrity of isolated RNA is assessed with bioanalyzer analysis, and the presence of mRNA of a specific gene is validated through RT-PCR. This RNA could further be used for uncovering single cell type-specific gene expression signature using next-generation transcriptome sequence or through regular RT-PCR. | en_US |
dc.description.sponsorship | V.G. and S.C. thank the Council of Scientific and Industrial Research (CSIR), India, and the National Institute of Plant Genome Research (NIPGR), New Delhi, India, for funding and internal grants. VG also acknowledges the Department of Biotechnology (DBT), India, for fellowship. A.K.S. thanks NIPGR and DBT (Project Grant No.BT/PR12766/BPA/118/63/2015), New Delhi, India, for fellowship and grants. The authors declare no conflict of interests. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature Publishing AG | en_US |
dc.subject | LCM | en_US |
dc.subject | Laser microdissection | en_US |
dc.subject | RNA isolation | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Mesophyll cells | en_US |
dc.subject | Bundle sheath cells | en_US |
dc.subject | Rice | en_US |
dc.title | Single cell type specific RNA isolation and gene expression analysis in rice using laser capture microdissection (LCM)-based method | en_US |
dc.type | Book chapter | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sarkar AK_2021_1.pdf Restricted Access | 380.31 kB | Adobe PDF | View/Open Request a copy |
Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.