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Title: | Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice |
Authors: | Jain, Mukesh Khurana, Jitendra P. |
Keywords: | abiotic stress auxin microarray analysis reproductive development rice (Oryza sativa) |
Issue Date: | 2009 |
Publisher: | Wiley-Blackwell |
Citation: | FEBS Journal, 276(11): 3148-3162 |
Abstract: | Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Arabidopsis in detail, but not in crop plants. Earlier, we reported the identification and characterization of the members of the GH3, Aux/IAA and SAUR gene families in rice. In this study, whole genome microarray analysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A comparison of expression profiles of untreated and auxin-treated rice seedlings identified 315 probe sets representing 298 (225 upregulated and 73 downregulated) unique genes as auxin-responsive. Functional categorization revealed that genes involved in various biological processes, including metabolism, transcription, signal transduction, and transport, are regulated by auxin. The expression profiles of auxin-responsive genes identified in this study and those of the members of the GH3, Aux/IAA, SAUR and ARF gene families were analyzed during various stages of vegetative and reproductive (panicle and seed) development by employing microarray analysis. Many of these genes are, indeed, expressed in a tissue-specific or developmental stage-specific manner, and the expression profiles of some of the representative genes were confirmed by real-time PCR. The differential expression of auxin-responsive genes during various stages of panicle and seed development implies their involvement in diverse developmental processes. Moreover, several auxin-responsive genes were differentially expressed under various abiotic stress conditions, indicating crosstalk between auxin and abiotic stress signaling. |
URI: | http://hdl.handle.net/123456789/116 |
Appears in Collections: | Institutional Publications |
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Jain M_2009_1.pdf Restricted Access | 1.57 MB | Adobe PDF | View/Open Request a copy |
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