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DC Field | Value | Language |
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dc.contributor.author | Bansal, Sakshi | - |
dc.contributor.author | Sardar, Shaswati | - |
dc.contributor.author | Sinha, Kshitija | - |
dc.contributor.author | Bhunia, Rupam Kumar | - |
dc.contributor.author | Katoch, Megha | - |
dc.contributor.author | Sonah, Humira | - |
dc.contributor.author | Deshmukh, Rupesh | - |
dc.contributor.author | Ram, Hasthi | - |
dc.date.accessioned | 2021-06-04T06:12:37Z | - |
dc.date.available | 2021-06-04T06:12:37Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Plant Cell Reports, 40(7): 1215-1228 | en_US |
dc.identifier.issn | 0721-7714 | - |
dc.identifier.other | https://doi.org/10.1007/s00299-021-02714-4 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00299-021-02714-4 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1187 | - |
dc.description | Accepted date: 15 May 2021 | en_US |
dc.description.abstract | Key message Among the 113 lipases present in rice genome, bran and endosperm-specifc lipases were identifed and lipase activity for one of the selected lipase gene is demonstrated in yeast. Abstract: Rice bran is nutritionally superior than endosperm as it has major reservoirs of various minerals, vitamins, essential mineral oils and other bioactive compounds, however it is often under-utilized as a food product due to bran instability after milling. Various hydrolytic enzymes, such as lipases, present in bran causes degradation of the lipids present and are responsible for the bran instability. Here, in this study, we have systematically analyzed the 113 lipase genes present in rice genome, and identifed 21 seed-specifc lipases. By analyzing the expression of these genes in diferent seed tissues during seed development, we have identifed three bran-specifc and three endosperm-specifc lipases, and one lipase which expresses in both bran and endosperm tissues. Further analysis of these genes during seed maturation and seed germination revealed that their expression increases during seed maturation and decreases during seed germination. Finally, we have shown the lipase activity for one of the selected genes, LOC_Os05g30900, in heterologous system yeast. The bran-specifc lipases identifed in this study would be very valuable for engineering designer rice varieties having increased bran stability in post-milling. | en_US |
dc.description.sponsorship | The funding was funded by DST-INSPIRE Faculty grant from Department of Science and Technology, Govt. of India (Grant no. DST/ INSPIRE/04/2016/001118). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature Publishing AG | en_US |
dc.subject | Rice | en_US |
dc.subject | Seed | en_US |
dc.subject | Bran | en_US |
dc.subject | Lipases | en_US |
dc.subject | Gene expression | en_US |
dc.subject | q-PCR | en_US |
dc.title | Identification and molecular characterization of rice bran-specific lipases | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Ram H_2021_4.pdf Restricted Access | 2.72 MB | Adobe PDF | View/Open Request a copy |
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