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http://223.31.159.10:8080/jspui/handle/123456789/1237
Title: | A sick plot-based protocol for dry root rot disease assessment in field-grown chickpea plants |
Authors: | Irulappan, Vadivelmurugan Mali, Komal Vitthalrao Patil, Basavanagouda S. Manjunatha, Hanumappa Muhammad, Saifulla Senthil-Kumar, Muthappa |
Keywords: | Cicer arietinum combined stress dry root rot Macrophomina phaseolina Rhizoctonia bataticola sick plot technique |
Issue Date: | 2021 |
Publisher: | John Wiley & Sons |
Citation: | Applications in Plant Sciences, 9(8): e11445 |
Abstract: | Premise: A comprehensive field-based screening protocol is lacking for dry root rot (DRR) disease in chickpea, which is caused by Macrophomina phaseolina (formerly referred to as Rhizoctonia bataticola). Here, we describe a protocol for establishing a sick plot for DRR to enable disease assessment of a large number of chickpea plants during the natural growing season. Methods and Results: We used a chickpea plot with >30% DRR incidence, and enriched the inoculum by cultivating highly susceptible chickpea plant genotypes and incorporating infected plant material into the soil. The chickpea plants were then subjected to infection in developed sick plots with various levels of soil moisture under natural field conditions. Conclusions: Our protocol provides a robust way to impose M. phaseolina infection on chickpea plants under natural field conditions and to investigate plant responses to the infection at morphological, physiological, and molecular levels. This method can also be used to screen for other soil‐borne diseases in a variety of plants. |
Description: | Accepted date: 21 July 2021 |
URI: | https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.11445 http://223.31.159.10:8080/jspui/handle/123456789/1237 |
ISSN: | 2168-0450 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Senthil-Kumar M_2021_6.pdf | 4.64 MB | Adobe PDF | ![]() View/Open |
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