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Title: | The sly-miR166-SlyHB module acts as a susceptibility factor during ToLCNDV infection |
Authors: | Prasad, Ashish Sharma, Namisha Chirom, Oceania Prasad, Manoj |
Keywords: | Tomato leaf curl New Delhi virus (ToLCNDV) miRNAs sly-miR166/SlyHB Solanum lycopersicum Tomato leaf curl New Delhi virus |
Issue Date: | 2022 |
Publisher: | Springer Nature Publishing AG |
Citation: | Theoretical and Applied Genetics, 135(1): 233-242 |
Abstract: | Tomato leaf curl New Delhi virus (ToLCNDV) is a devastating pathogen that causes huge crop loss. It is spreading to new geographical locations at a very rapid rate-raising serious concerns. Evolution of insecticidal resistance in Bemisia tabaci which acts as the carrier for ToLCNDV has made insect control very difficult in the recent years. Thus, it is important that the host molecular mechanisms associated with ToLCNDV resistance/susceptibility are investigated to develop management strategies. In our study, we have identified that sly-miR166/SlyHB module acts as a susceptibility factor to ToLCNDV in Solanum lycopersicum. Sly-miR166 is differentially regulated upon ToLCNDV infection in two contrasting tomato cultivars; H-88-78-1 (tolerant to ToLCNDV) and Punjab Chhuhara (susceptible to ToLCNDV). Expression analysis of predicted sly-miR166 targets revealed that the expression of SlyHB is negatively correlated with its corresponding miRNA. Virus-induced gene silencing of SlyHB in the susceptible tomato cultivar resulted in the decrease in disease severity suggesting that SlyHB is a negative regulator of plant defence. In summary, our study highlights a miRNA/target module that acts as a susceptibility factor during ToLCNDV infection. To the best of our knowledge, this is the first report that highlights the role of sly-miR166/SlyHB module in ToLCNDV pathogenesis. |
Description: | Accepted date: 29 September 2021 |
URI: | https://link.springer.com/article/10.1007%2Fs00122-021-03962-4 http://223.31.159.10:8080/jspui/handle/123456789/1248 |
ISSN: | 0040-5752 |
Appears in Collections: | Institutional Publications |
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Prasad M_2022_3.pdf Restricted Access | 1.67 MB | Adobe PDF | View/Open Request a copy |
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