Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/1251
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Raul, Bikash | - |
dc.contributor.author | Bhattacharjee, Oindrila | - |
dc.contributor.author | Ghosh, Amit | - |
dc.contributor.author | Upadhyay, Priya | - |
dc.contributor.author | Tembhare, Kunal | - |
dc.contributor.author | Singh, Ajeet | - |
dc.contributor.author | Shaheen, Tarannum | - |
dc.contributor.author | Ghosh, Asim Kumar | - |
dc.contributor.author | Torres-Jerez, Ivone | - |
dc.contributor.author | Krom, Nick | - |
dc.contributor.author | Clevenger, Josh | - |
dc.contributor.author | Udvardi, Michael | - |
dc.contributor.author | Scheffler, Brian E. | - |
dc.contributor.author | Ozias-Akins, Peggy | - |
dc.contributor.author | Sharma, Ravi Datta | - |
dc.contributor.author | Bandyopadhyay, Kaustav | - |
dc.contributor.author | Gaur, Vineet | - |
dc.contributor.author | Kumar, Shailesh | - |
dc.contributor.author | Sinharoy, Senjuti | - |
dc.date.accessioned | 2021-11-03T06:26:16Z | - |
dc.date.available | 2021-11-03T06:26:16Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Molecular Plant-Microbe Interactions, 35(2): 131-145 | en_US |
dc.identifier.issn | 0894-0282 | - |
dc.identifier.other | https://doi.org/10.1094/MPMI-05-21-0122-R | - |
dc.identifier.uri | https://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-05-21-0122-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1251 | - |
dc.description | Accepted date: 23 Oct 2021 | en_US |
dc.description.abstract | Root nodule symbiosis (RNS) is the pillar behind sustainable agriculture and plays a pivotal role in the environmental nitrogen cycle. Most of the genetic, molecular, and cell-biological knowledge on RNS come from model legumes that exhibit a root-hair mode of bacterial infection in contrast to the Dalbergoid legumes exhibiting crack-entry of rhizobia. As a step towards understanding this important group of legumes, we have combined microscopic analysis and temporal transcriptome to obtain a dynamic view of plant gene expression during Arachis hypogaea (peanut) nodule development. We generated a comprehensive transcriptome data by mapping the reads to A. hypogaea, and two diploid progenitor genomes. Additionally, we performed BLAST searches to identify nodule-induced yet-to-be annotated peanut genes. Comparison between peanut, Medicago truncatula, Lotus japonicus, and Glycine max showed upregulation of 61 peanut orthologs among 111 tested known RNS-related genes, indicating conservation in mechanisms of nodule development among members of the Papilionoid family. Unlike model legumes, recruitment of class 1 phytoglobin derived symbiotic hemoglobin (SymH) in peanut indicates diversification of oxygen scavenging mechanisms in the Papilionoid family. Finally, absence of cysteine-rich motif-1 containing-NCRs, but the recruitment of defensin like NCRs suggest a diverse molecular mechanism of terminal bacteroid differentiation. In summary, our work describes genetic conservation and diversification in legume-rhizobial symbiosis in the Papilionoid family, as well as among members of the Dalbergoid legumes. | en_US |
dc.description.sponsorship | We thank Pasupuleti, J. and Azevedo, V. ICRISAT, India for A. hypogaea Tifrunner seeds for the experiments performed in India; Ibáñez, F., Universidad Nacional de Río Cuarto, Argentina, for Bradyrhizobium sp. SEMIA 6144, NIPGR for confocal facilities, Bhardwaj, A. for cloning peanut RSD; CIF-NIPGR and DBT (Department of Biotechnology)-eLibrary Consortium (DeLCON), India for providing access to e-resources. Work was supported by core grants from NIPGR, Ramalingaswami Re-entry grant (BT/RLF/Re- entry/41/2013), and DBT grant BT/PR23489/BPA/118/287/2017, Peanut Foundation, funding provided to BES from MARs INC (58-6402-2-723). Bikash Raul was supported by CSIR (09/803(0141)/2017-EMR-I). Work in the Udvardi lab was supported by a grant from the US National Science Foundation (grant # DBI-1733470). The complete transcriptome data is submitted to NCBI (Accession ID: PRJNA596350). Additionally, the data is available in (http://nipgr.ac.in/AhNGE/index.php). AhRSD sequence is submitted to NCBI (MW848437). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Phytopathological Society | en_US |
dc.subject | Peanut | en_US |
dc.subject | Arachis hypogaea | en_US |
dc.subject | nodule development | en_US |
dc.subject | root nodule symbiosis | en_US |
dc.subject | nitrogen fixation | en_US |
dc.subject | bacteroid | en_US |
dc.subject | Nod Factors | en_US |
dc.subject | Dalbergoid legumes | en_US |
dc.subject | crack entry | en_US |
dc.title | Microscopic and transcriptomic analyses of Dalbergoid legume peanut reveal a divergent evolution leading to Nod Factor dependent epidermal crack-entry and terminal bacteroid differentiation | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sinharoy S_2022_1.pdf | 999.84 kB | Adobe PDF | View/Open |
Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.