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Title: | Microscopic and transcriptomic analyses of Dalbergoid legume peanut reveal a divergent evolution leading to Nod Factor dependent epidermal crack-entry and terminal bacteroid differentiation |
Authors: | Raul, Bikash Bhattacharjee, Oindrila Ghosh, Amit Upadhyay, Priya Tembhare, Kunal Singh, Ajeet Shaheen, Tarannum Ghosh, Asim Kumar Torres-Jerez, Ivone Krom, Nick Clevenger, Josh Udvardi, Michael Scheffler, Brian E. Ozias-Akins, Peggy Sharma, Ravi Datta Bandyopadhyay, Kaustav Gaur, Vineet Kumar, Shailesh Sinharoy, Senjuti |
Keywords: | Peanut Arachis hypogaea nodule development root nodule symbiosis nitrogen fixation bacteroid Nod Factors Dalbergoid legumes crack entry |
Issue Date: | 2022 |
Publisher: | American Phytopathological Society |
Citation: | Molecular Plant-Microbe Interactions, 35(2): 131-145 |
Abstract: | Root nodule symbiosis (RNS) is the pillar behind sustainable agriculture and plays a pivotal role in the environmental nitrogen cycle. Most of the genetic, molecular, and cell-biological knowledge on RNS come from model legumes that exhibit a root-hair mode of bacterial infection in contrast to the Dalbergoid legumes exhibiting crack-entry of rhizobia. As a step towards understanding this important group of legumes, we have combined microscopic analysis and temporal transcriptome to obtain a dynamic view of plant gene expression during Arachis hypogaea (peanut) nodule development. We generated a comprehensive transcriptome data by mapping the reads to A. hypogaea, and two diploid progenitor genomes. Additionally, we performed BLAST searches to identify nodule-induced yet-to-be annotated peanut genes. Comparison between peanut, Medicago truncatula, Lotus japonicus, and Glycine max showed upregulation of 61 peanut orthologs among 111 tested known RNS-related genes, indicating conservation in mechanisms of nodule development among members of the Papilionoid family. Unlike model legumes, recruitment of class 1 phytoglobin derived symbiotic hemoglobin (SymH) in peanut indicates diversification of oxygen scavenging mechanisms in the Papilionoid family. Finally, absence of cysteine-rich motif-1 containing-NCRs, but the recruitment of defensin like NCRs suggest a diverse molecular mechanism of terminal bacteroid differentiation. In summary, our work describes genetic conservation and diversification in legume-rhizobial symbiosis in the Papilionoid family, as well as among members of the Dalbergoid legumes. |
Description: | Accepted date: 23 Oct 2021 |
URI: | https://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-05-21-0122-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed http://223.31.159.10:8080/jspui/handle/123456789/1251 |
ISSN: | 0894-0282 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sinharoy S_2022_1.pdf | 999.84 kB | Adobe PDF | View/Open |
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