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http://223.31.159.10:8080/jspui/handle/123456789/1286
Title: | Cultivar-biased regulation of HSFA7 and HSFB4a govern high-temperature tolerance in tomato |
Authors: | Rao, Sombir Das, Jaishri Rubina Balyan, Sonia Verma, Radhika Mathur, Saloni |
Keywords: | Heat Heat shock factor Genetic variation Solanum lycopersicum Thermo-tolerance Tomato cv CLN, CA4, Pusa Ruby |
Issue Date: | 2022 |
Publisher: | Springer Nature Publishing AG |
Citation: | Planta, 255(2): 31 |
Abstract: | Heat shock factors (HSFs) are at the core of heat stress (HS) response in plants. However, the contribution of HSFs governing the inherent thermo-tolerance mechanism in tomato from sub-tropical hot climates is poorly understood. With the above aim, comparative expression profiles of the HSF family in a HS-tolerant (CLN1621L) and -sensitive cultivars (CA4 and Pusa Ruby) of tomato under HS revealed cultivar-biased regulation of an activator (HSFA7) and a repressor (HSFB4a) class HSF. HSFA7 exhibited strong upregulation while HSFB4a showed downregulation in tolerant tomato cultivar upon HS. Functional characterization of HSFA7 and HSFB4a in a tolerant–sensitive cultivar pair by virus-induced gene silencing (VIGS)-based silencing and transient overexpression established them as a positive and a negative regulator of HS tolerance, respectively. Promoter:GUS reporter assays and promoter sequence analyses suggest heat-mediated transcriptional control of both the HSF genes in the contrasting cultivars. Moreover, degradome data highlighted HSFB4a is a probable target of microRNA Sly-miR4200. Transient in-planta Sly-MIR4200-effector:HSFB4a-reporter assays showed miRNA-dependent target down-regulation. Chelation of miRNA by short-tandem-target-mimic of Sly-miR4200 increased target abundance, highlighting a link between Sly-miR4200 and HSFB4a. This miRNA has induced several folds upon HS in the tolerant cultivar where HSFB4a levels are reduced, thus exhibiting the inverse miR:target expression. Thus, we speculate that the alleviation of HSFB4a and increased HSFA7 levels govern thermo-tolerance in the tolerant cultivar by regulating downstream heat stress-responsive genes. |
Description: | Accepted date: 17 December 2021 |
URI: | https://link.springer.com/article/10.1007%2Fs00425-021-03813-y http://223.31.159.10:8080/jspui/handle/123456789/1286 |
ISSN: | 0032-0935 1432-2048 |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Mathur S_2022_1.pdf Restricted Access | 4.99 MB | Adobe PDF | View/Open Request a copy |
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