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Title: | A unique miR775-GALT9 module regulates leaf senescence in Arabidopsis during post-submergence recovery by modulating ethylene and the abscisic acid pathway |
Authors: | Mishra, Vishnu Singh, Archita Gandhi, Nidhi Das, Shabari Sarkar Yadav, Sandeep Kumar, Ashutosh Sarkar, Ananda K. |
Keywords: | MiRNA MiR775 GALT9 Senescence Submergence stress Hypoxia SAGs Arabidopsis |
Issue Date: | 2022 |
Publisher: | The Company of Biologists |
Citation: | Development, 149(4): dev199974 |
Abstract: | Submergence-induced hypoxic condition negatively affects the plant growth and development, and causes early onset of senescence. Hypoxia alters the expression of a number of microRNAs (miRNAs). However, the molecular function of submergence stress-induced miRNAs in physiological or developmental changes and recovery remains poorly understood. Here we show that miR775 is an Arabidopsis thaliana-specific young and unique miRNA that possibly evolved non-canonically. miR775 post-transcriptionally regulates Galactosyltransferase (GALT9) and their expression is inversely affected at 24 hours of complete submergence stress. The overexpression of miR775 (miR775-Oe) confers enhanced recovery from submergence stress and reduced accumulation of RBOHD and ROS, in contrast to wild type and MIM775 Arabidopsis shoot. A similar recovery phenotype of galt9 mutant indicates the role of miR775-GALT9 module in post-submergence recovery. We predicted Golgi-localized GALT9 to be potentially involved in protein glycosylation. The altered expression of senescence-associated genes (SAG12, SAG29, and ORE1), ethylene signalling (EIN2 and EIN3) and ABA biosynthesis (NCED3) pathway genes in miR775-Oe, galt9 and MIM775 plants. Thus, our results indicate the role of miR775-GALT9 module in post-submergence recovery through a crosstalk with ethylene and ABA pathway. |
Description: | Accepted date: 06 Dec 2021 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/1289 https://journals.biologists.com/dev/article/doi/10.1242/dev.199974/274011/Unique-miR775-GALT9-module-regulates-leaf |
ISSN: | 1477-9129 0950-1991 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sarkar AK_2022_1.pdf Restricted Access | 4.65 MB | Adobe PDF | View/Open Request a copy |
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