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DC Field | Value | Language |
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dc.contributor.author | Mishra, Vishnu | - |
dc.contributor.author | Singh, Archita | - |
dc.contributor.author | Gandhi, Nidhi | - |
dc.contributor.author | Das, Shabari Sarkar | - |
dc.contributor.author | Yadav, Sandeep | - |
dc.contributor.author | Kumar, Ashutosh | - |
dc.contributor.author | Sarkar, Ananda K. | - |
dc.date.accessioned | 2022-01-25T09:13:46Z | - |
dc.date.available | 2022-01-25T09:13:46Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Development, 149(4): dev199974 | en_US |
dc.identifier.issn | 1477-9129 | - |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1289 | - |
dc.identifier.uri | https://journals.biologists.com/dev/article/doi/10.1242/dev.199974/274011/Unique-miR775-GALT9-module-regulates-leaf | - |
dc.description | Accepted date: 06 Dec 2021 | en_US |
dc.description.abstract | Submergence-induced hypoxic condition negatively affects the plant growth and development, and causes early onset of senescence. Hypoxia alters the expression of a number of microRNAs (miRNAs). However, the molecular function of submergence stress-induced miRNAs in physiological or developmental changes and recovery remains poorly understood. Here we show that miR775 is an Arabidopsis thaliana-specific young and unique miRNA that possibly evolved non-canonically. miR775 post-transcriptionally regulates Galactosyltransferase (GALT9) and their expression is inversely affected at 24 hours of complete submergence stress. The overexpression of miR775 (miR775-Oe) confers enhanced recovery from submergence stress and reduced accumulation of RBOHD and ROS, in contrast to wild type and MIM775 Arabidopsis shoot. A similar recovery phenotype of galt9 mutant indicates the role of miR775-GALT9 module in post-submergence recovery. We predicted Golgi-localized GALT9 to be potentially involved in protein glycosylation. The altered expression of senescence-associated genes (SAG12, SAG29, and ORE1), ethylene signalling (EIN2 and EIN3) and ABA biosynthesis (NCED3) pathway genes in miR775-Oe, galt9 and MIM775 plants. Thus, our results indicate the role of miR775-GALT9 module in post-submergence recovery through a crosstalk with ethylene and ABA pathway. | en_US |
dc.description.sponsorship | We acknowledge NIPGR for providing necessary research facilities (plant growth facility, confocal/other microscopic facility, other central instrument facility) and internal grants. We acknowledge the Department of Biotechnology (DBT), Govt. of India for financial support (BT/PR12766/BPA/188/63/2015) and fellowship to VM (DBT/JRF/15/AL/223). We also acknowledge DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. AKS acknowledges support from NIPGR and SLS, Jawaharlal Nehru University. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | The Company of Biologists | en_US |
dc.subject | MiRNA | en_US |
dc.subject | MiR775 | en_US |
dc.subject | GALT9 | en_US |
dc.subject | Senescence | en_US |
dc.subject | Submergence stress | en_US |
dc.subject | Hypoxia | en_US |
dc.subject | SAGs | en_US |
dc.subject | Arabidopsis | en_US |
dc.title | A unique miR775-GALT9 module regulates leaf senescence in Arabidopsis during post-submergence recovery by modulating ethylene and the abscisic acid pathway | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Sarkar AK_2022_1.pdf Restricted Access | 4.65 MB | Adobe PDF | View/Open Request a copy |
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