Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1313
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dc.contributor.authorYamunarani, Ramegowda-
dc.contributor.authorRamegowda, Venkategowda-
dc.contributor.authorSenthil-Kumar, Muthappa-
dc.contributor.authorMysore, Kirankumar S.-
dc.date.accessioned2022-04-01T06:28:00Z-
dc.date.available2022-04-01T06:28:00Z-
dc.date.issued2022-
dc.identifier.citationMethods in Molecular Biology, 2408: 181-189en_US
dc.identifier.isbn978-1-0716-1875-2-
dc.identifier.isbn978-1-0716-1874-5-
dc.identifier.otherhttps://doi.org/10.1007/978-1-0716-1875-2_12-
dc.identifier.urihttps://link.springer.com/protocol/10.1007/978-1-0716-1875-2_12-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/1313-
dc.descriptionAccepted date: 25 March 2022en_US
dc.description.abstractThe high throughputness and affordability of “omics” technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo functional characterization of these genes under multiple stresses is challenging but essential to develop resilient crops for the changing climate. Here we describe a high-throughput Virus-Induced Gene Silencing-based methodology for functional analysis of genes under multiple abiotic stresses using leaf disks. Leaves with maximal silencing, which is localized to only a few leaves and to a short period, can be effectively used for multiple stress imposition and stress affect quantification.en_US
dc.description.sponsorshipRV thanks SERB, DST, Govt. of India for Ramanujan Fellowship (SE/S2/RJN-039/2016) and Early Career Research Award (ECR/2018/00l942).en_US
dc.language.isoen_USen_US
dc.publisherSpringer Nature Publishing AGen_US
dc.subjectVirus-Induced Gene Silencingen_US
dc.subjectLeaf disksen_US
dc.subjectMultiple abiotic stressesen_US
dc.titleHigh-throughput analysis of gene function under multiple abiotic stresses using leaf disks from silenced plantsen_US
dc.typeBook chapteren_US
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