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http://223.31.159.10:8080/jspui/handle/123456789/1317
Title: | De novo transcriptome analysis identifies key genes involved in dehydration stress response in kodo millet (Paspalum scrobiculatum L.) |
Authors: | Suresh, Bonthala Venkata Choudhary, Pooja Aggarwal, Pooja Rani Rana, Sumi Singh, Roshan Kumar Ravikesavan, Rajasekaran Prasad, Manoj Muthamilarasan, Mehanathan |
Keywords: | Kodo millet (Paspalum scrobiculatum L.) RNA sequencing Dehydration stress Functional annotation Gene expression |
Issue Date: | 2022 |
Publisher: | Elsevier B.V. |
Citation: | Genomics, 114(3): 110347 |
Abstract: | Kodo millet (Paspalum scrobiculatum L.) is a small millet species known for its excellent nutritional and climate-resilient traits. To understand the genes and pathways underlying dehydration stress tolerance of kodo millet, the transcriptome of cultivar ‘CO3’ subjected to dehydration stress (0 h, 3 h, and 6 h) was sequenced. The study generated 239.1 million clean reads that identified 9201, 9814, and 2346 differentially expressed genes (DEGs) in 0 h vs. 3 h, 0 h vs. 6 h, and 3 h vs. 6 h libraries, respectively. The DEGs were found to be associated with vital molecular pathways, including hormone metabolism and signaling, antioxidant scavenging, photosynthesis, and cellular metabolism, and were validated using qRT-PCR. Also, a higher abundance of uncharacterized genes expressed during stress warrants further studies to characterize this class of genes to understand their role in dehydration stress response. Altogether, the study provides insights into the transcriptomic response of kodo millet during dehydration stress. |
Description: | Accepted date: 18 March 2022 |
URI: | https://www.sciencedirect.com/science/article/pii/S0888754322000921?via%3Dihub http://223.31.159.10:8080/jspui/handle/123456789/1317 |
ISSN: | 0888-7543 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Prasad M_2022_4.pdf | 8.42 MB | Adobe PDF | View/Open |
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