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DC Field | Value | Language |
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dc.contributor.author | Saini, Kumud | - |
dc.contributor.author | Dwivedi, Aditi | - |
dc.contributor.author | Ranjan, Aashish | - |
dc.date.accessioned | 2022-07-28T07:09:27Z | - |
dc.date.available | 2022-07-28T07:09:27Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Plant Physiology, 190(4): 2380-2397 | en_US |
dc.identifier.issn | 1532-2548 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.other | https://doi.org/10.1093/plphys/kiac345 | - |
dc.identifier.uri | https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiac345/6650114 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1374 | - |
dc.description | Accepted date: 30 June 2022 | en_US |
dc.description.abstract | High ambient temperature suppresses Arabidopsis (Arabidopsis thaliana) rosette leaf area and elongates the stem and petiole. While the mechanism underlying the temperature-induced elongation response has been extensively studied, the genetic basis of temperature regulation of leaf size is largely unknown. Here, we show that warm temperature inhibits cell proliferation in Arabidopsis leaves, resulting in fewer cells compared to the control condition. Cellular phenotyping and genetic and biochemical analyses established the key roles of PHYTOCHROME INTERACTING FACTOR4 (PIF4) and TEOSINTE BRANCHED1/CYCLOIDEA/PCF4 (TCP4) transcription factors in the suppression of Arabidopsis leaf area under high temperature by a reduction in cell number. We show that temperature-mediated suppression of cell proliferation requires PIF4, which interacts with TCP4 and regulates the expression of the cell cycle inhibitor KIP-RELATED PROTEIN1 (KRP1) to control leaf size under high temperature. Warm temperature induces binding of both PIF4 and TCP4 to the KRP1 promoter. PIF4 binding to KRP1 under high temperature is TCP4 dependent as TCP4 regulates PIF4 transcript levels under high temperature. We propose a model where a warm temperature-mediated accumulation of PIF4 in leaf cells promotes its binding to the KRP1 promoter in a TCP4-dependent way to regulate cell production and leaf size. Our finding of high-temperature-mediated transcriptional upregulation of KRP1 integrates a developmental signal with an environmental signal that converges on a basal cell regulatory process. | en_US |
dc.description.sponsorship | We are grateful to Gerrit Beemster for sharing with us the seeds of krp1, and krp4/6/7, Christian Fankhauser for pif1345, Utpal Nath for tcp4-2 and pTCP4:GUS lines, On Sun Lau for pPIF4:PIF4-GFP, and to Takato Imaizumi for the 35S::TCP797 3F6H line. We thank Praveen Verma for providing the p635nRRF vector for dual luciferase assay. K.S. acknowledge her DBT-RA and SERB-NPDF fellowship, and A.D. acknowledge her UGC-JRF fellowship. We thank the Confocal facility and other Central Instrumentation Facility (CIF) at NIPGR for their support. This work was supported by Ramalingaswamy Re-entry Fellowship (BT/RLF/Re788 entry/05/2013) from the Department of Biotechnology, Ministry of Science and Technology, India, and SERB-Core Research Grant (CRG/2020/002179) and SERB NPDF scheme (PDF/2018/002387) from Science and Engineering Research Board, India. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Oxford University Press | en_US |
dc.subject | Cell cycle | en_US |
dc.subject | KRP1 | en_US |
dc.subject | PIF4 | en_US |
dc.subject | TCP4 | en_US |
dc.subject | cell division | en_US |
dc.subject | high temperature | en_US |
dc.subject | leaf size | en_US |
dc.title | High temperature restricts cell division and leaf size by coordination of PIF4 and TCP4 transcription factors | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Ranjan A_2022_2.pdf Restricted Access | 1.86 MB | Adobe PDF | View/Open Request a copy |
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