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DC Field | Value | Language |
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dc.contributor.author | Jindal, Sunita | - |
dc.contributor.author | Kerchev, Pavel | - |
dc.contributor.author | Berka, Miroslav | - |
dc.contributor.author | Černý, Martin | - |
dc.contributor.author | Botta, Halidev Krishna | - |
dc.contributor.author | Laxmi, Ashverya | - |
dc.contributor.author | Brzobohatý, Břetislav | - |
dc.date.accessioned | 2022-10-14T06:27:31Z | - |
dc.date.available | 2022-10-14T06:27:31Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Frontiers in Plant Science, 13: 968139 | en_US |
dc.identifier.issn | 1664-462X | - |
dc.identifier.other | https://doi.org/10.3389/fpls.2022.968139 | - |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fpls.2022.968139/full | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1409 | - |
dc.description | Accepted date: 02 September 2022 | en_US |
dc.description.abstract | Besides the long-standing role of cytokinins (CKs) as growth regulators, their current positioning at the interface of development and stress responses is coming into recognition. The current evidence suggests the notion that CKs are involved in heat stress response (HSR), however, the role of CK signaling components is still elusive. In this study, we have identified a role of the CK signaling components type-A Arabidopsis response regulators (ARRs) in HSR in Arabidopsis. The mutants of multiple type-A ARR genes exhibit improved basal and acquired thermotolerance and, altered response to oxidative stress in our physiological analyses. Through proteomics profiling, we show that the type-A arr mutants experience a 'stress-primed' state enabling them to respond more efficiently upon exposure to real stress stimuli. A substantial number of proteins that are involved in the heat-acclimatization process such as the proteins related to cellular redox status and heat shock, are already altered in the type-A arr mutants without a prior exposure to stress conditions. The metabolomics analyses further reveal that the mutants accumulate higher amounts of α-and γ-tocopherols, which are important antioxidants for protection against oxidative damage. Collectively, our results suggest that the type-A ARRs play an important role in heat stress response by affecting the redox homeostasis in Arabidopsis. | en_US |
dc.description.sponsorship | This work was supported by Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project “Centre for Experimental Plant Biology” (Grant no. CZ.02.1.01/0.0/0.0/16_019/0000738) and the Internal Grant Schemes of Mendel University in Brno. Reg. no. CZ.02.2.69/0.0/0.0/19_073/0016670, funded by the ESF. SJ, BB, and AL conceived the study. SJ, BB, and PK designed experiments. SJ and HKB performed thermotolerance assays. SJ, PK, and MČ performed proteomics studies and analysed data. SJ, PK, and MB performed metabolomics study. SJ performed all other experiments. SJ wrote the paper and BB and MČ reviewed the paper. BB and SJ revised the manuscript. BB acquired funding. All authors have read and agreed to the published version of the manuscript. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Frontiers Media S.A. | en_US |
dc.subject | Arabidopsis response regulators | en_US |
dc.subject | heat stress | en_US |
dc.subject | cytokinins | en_US |
dc.subject | oxidative stress | en_US |
dc.subject | proteomics | en_US |
dc.subject | metabolomics | en_US |
dc.subject | heat-acclimatization | en_US |
dc.title | Type-A response regulators negatively mediate heat stress response by altering redox homeostasis in Arabidopsis | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Laxmi A_2022_5.pdf Restricted Access | 11.87 MB | Adobe PDF | View/Open Request a copy |
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