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http://223.31.159.10:8080/jspui/handle/123456789/1447
Title: | miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana |
Authors: | Lu, Lu Holt, Anna Chen, Xinying Liu, Yang Knauer, Steffen Tucker, Elise J. Sarkar, Ananda K. Hao, Zhaodong Roodbarkelari, Farshad Shi, Jisen Chen, Jinhui Laux, Thomas |
Keywords: | miR394 WUSCHEL LEAF CURLING RESPONSIVENESS somatic embryogenesis pluripotency ecotype |
Issue Date: | 2023 |
Publisher: | John Wiley & Sons |
Citation: | New Phytologist, 238(3): 1059-1072 |
Abstract: | Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive. We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394-enhanced somatic embryogenesis. We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co-expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared to Ler, providing a mechanistic explanation for this natural variation. Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS-mediated direct transcriptional repression. |
Description: | Manuscript Accepted: 30 January 2023 |
URI: | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18801 http://223.31.159.10:8080/jspui/handle/123456789/1447 |
ISSN: | 1469-8137 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sarkar AK_2023_1.pdf Restricted Access | 2.35 MB | Adobe PDF | View/Open Request a copy |
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