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DC Field | Value | Language |
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dc.contributor.author | Lu, Lu | - |
dc.contributor.author | Holt, Anna | - |
dc.contributor.author | Chen, Xinying | - |
dc.contributor.author | Liu, Yang | - |
dc.contributor.author | Knauer, Steffen | - |
dc.contributor.author | Tucker, Elise J. | - |
dc.contributor.author | Sarkar, Ananda K. | - |
dc.contributor.author | Hao, Zhaodong | - |
dc.contributor.author | Roodbarkelari, Farshad | - |
dc.contributor.author | Shi, Jisen | - |
dc.contributor.author | Chen, Jinhui | - |
dc.contributor.author | Laux, Thomas | - |
dc.date.accessioned | 2023-02-14T07:05:29Z | - |
dc.date.available | 2023-02-14T07:05:29Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | New Phytologist, 238(3): 1059-1072 | en_US |
dc.identifier.issn | 1469-8137 | - |
dc.identifier.other | https://doi.org/10.1111/nph.18801 | - |
dc.identifier.uri | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18801 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1447 | - |
dc.description | Manuscript Accepted: 30 January 2023 | en_US |
dc.description.abstract | Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive. We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394-enhanced somatic embryogenesis. We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co-expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared to Ler, providing a mechanistic explanation for this natural variation. Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS-mediated direct transcriptional repression. | en_US |
dc.description.sponsorship | We are grateful to Sascha Biedermann for comments on the manuscript and to Edwin Groot for statistical support. This work was funded by grants from the German Research Foundation (DFG) under Germany's Excellence Strategy (CIBSS - EXC-2189 - Project ID390939984), GRK2344, and by grants (La606/14, La606/17; La606/20) to T.L, the Key Research and Development Plan of Jiangsu Province (BE2017376), the Qinglan Project of Jiangsu province, the Distinguished Professor Project of Jiangsu province, the Doctorate Fellowship Foundation of Nanjing Forestry University (163010107), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). A.H. established the pOp:WUS and pOp:MIR394B/WUS lines and analyzed reprogramming, X.C. and Y.L. carried out miR394/WUS-GR induced SE formation at different stages, S.K. established the mir394b-1 line, E.J.T. made 35S:WUS-GR and 35S:MIR394B/35S:WUS-GR lines, Z.H. performed bioinformatic analysis, A.K.S, performed embryo analysis, and F.R. designed and analyzed the DEX treatment in somatic embryogenesis. L.L. carried out all other experiments, imaging, and analyses. J.S., J.C., and T.L. designed experiments, analyzed the data, and wrote the paper with input from all other authors | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | John Wiley & Sons | en_US |
dc.subject | miR394 | en_US |
dc.subject | WUSCHEL | en_US |
dc.subject | LEAF CURLING RESPONSIVENESS | en_US |
dc.subject | somatic embryogenesis | en_US |
dc.subject | pluripotency | en_US |
dc.subject | ecotype | en_US |
dc.title | miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Sarkar AK_2023_1.pdf Restricted Access | 2.35 MB | Adobe PDF | View/Open Request a copy |
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