Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1512
Title: A prophage tail-like protein facilitates the endophytic growth of Burkholderia gladioli and mounting immunity in tomato
Authors: Chandan, Ravindra Kumar
Kumar, Rahul
Kabyashree, Kristi
Yadav, Sunil Kumar
Roy, Mandira
Swain, Durga Madhab
Jha, Gopaljee
Keywords: FLS2
MAMP
T3SS
bacterial wilt disease
basal defense
endophytes
flg22
sheath blight disease
Issue Date: 2023
Publisher: John Wiley & Sons
Citation: New Phytologist, 240(3): 1202-1218
Abstract: A prophage tail-like protein (Bg_9562) of Burkholderia gladioli strain NGJ1 possesses broad-spectrum antifungal activity, and it is required for the bacterial ability to forage over fungi. Here, we analyzed whether heterologous overexpression of Bg_9562 or exogenous treatment with purified protein can impart disease tolerance in tomato. The physiological relevance of Bg_9562 during endophytic growth of NGJ1 was also investigated. Bg_9562 overexpressing lines demonstrate fungal and bacterial disease tolerance. They exhibit enhanced expression of defense genes and activation of mitogen-activated protein kinases. Treatment with Bg_9562 protein induces defense responses and imparts immunity in wild-type tomato. The defense-inducing ability lies within 18-51 aa region of Bg_9562 and is due to sequence homology with the bacterial flagellin epitope. Interaction studies suggest that Bg_9562 is perceived by FLAGELLIN-SENSING 2 homologs in tomato. The silencing of SlSERK3s (BAK1 homologs) prevents Bg_9562-triggered immunity. Moreover, type III secretion system-dependent translocation of Bg_9562 into host apoplast is important for elicitation of immune responses during colonization of NGJ1. Our study emphasizes that Bg_9562 is important for the endophytic growth of B. gladioli, while the plant perceives it as an indirect indicator of the presence of bacteria to mount immune responses. The findings have practical implications for controlling plant diseases.
Description: Accepted date: 19 July 2023
URI: https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19184
http://223.31.159.10:8080/jspui/handle/123456789/1512
ISSN: 1469-8137
Appears in Collections:Institutional Publications

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