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DC Field | Value | Language |
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dc.contributor.author | Chandan, Ravindra Kumar | - |
dc.contributor.author | Kumar, Rahul | - |
dc.contributor.author | Kabyashree, Kristi | - |
dc.contributor.author | Yadav, Sunil Kumar | - |
dc.contributor.author | Roy, Mandira | - |
dc.contributor.author | Swain, Durga Madhab | - |
dc.contributor.author | Jha, Gopaljee | - |
dc.date.accessioned | 2023-08-14T10:28:13Z | - |
dc.date.available | 2023-08-14T10:28:13Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | New Phytologist, 240(3): 1202-1218 | en_US |
dc.identifier.issn | 1469-8137 | - |
dc.identifier.other | https://doi.org/10.1111/nph.19184 | - |
dc.identifier.uri | https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19184 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/1512 | - |
dc.description | Accepted date: 19 July 2023 | en_US |
dc.description.abstract | A prophage tail-like protein (Bg_9562) of Burkholderia gladioli strain NGJ1 possesses broad-spectrum antifungal activity, and it is required for the bacterial ability to forage over fungi. Here, we analyzed whether heterologous overexpression of Bg_9562 or exogenous treatment with purified protein can impart disease tolerance in tomato. The physiological relevance of Bg_9562 during endophytic growth of NGJ1 was also investigated. Bg_9562 overexpressing lines demonstrate fungal and bacterial disease tolerance. They exhibit enhanced expression of defense genes and activation of mitogen-activated protein kinases. Treatment with Bg_9562 protein induces defense responses and imparts immunity in wild-type tomato. The defense-inducing ability lies within 18-51 aa region of Bg_9562 and is due to sequence homology with the bacterial flagellin epitope. Interaction studies suggest that Bg_9562 is perceived by FLAGELLIN-SENSING 2 homologs in tomato. The silencing of SlSERK3s (BAK1 homologs) prevents Bg_9562-triggered immunity. Moreover, type III secretion system-dependent translocation of Bg_9562 into host apoplast is important for elicitation of immune responses during colonization of NGJ1. Our study emphasizes that Bg_9562 is important for the endophytic growth of B. gladioli, while the plant perceives it as an indirect indicator of the presence of bacteria to mount immune responses. The findings have practical implications for controlling plant diseases. | en_US |
dc.description.sponsorship | MR and SKY acknowledge fellowship from CSIR and DBT, Govt of India, respectively. The authors are also thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. We are grateful to Dr Ramesh Sonti, ICGEB, New Delhi for providing critical comments on the manuscript. We acknowledge Dr Ramu S. Vemanna, RCB, Faridabad, for providing anti-FNR antibody. The research is supported by a Swarna Jayanti fellowship (SB/SJF/2020-21/01) from the Science and Engineering Research Board (SERB), Govt of India. Research funding from the Department of Biotechnology (DBT), Govt of India under the NIPGR Flagship program (imparting sheath blight disease tolerance in rice; 102/IFD/SAN/763/2019-20) and NIPGR core research grant are highly acknowledged. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | John Wiley & Sons | en_US |
dc.subject | FLS2 | en_US |
dc.subject | MAMP | en_US |
dc.subject | T3SS | en_US |
dc.subject | bacterial wilt disease | en_US |
dc.subject | basal defense | en_US |
dc.subject | endophytes | en_US |
dc.subject | flg22 | en_US |
dc.subject | sheath blight disease | en_US |
dc.title | A prophage tail-like protein facilitates the endophytic growth of Burkholderia gladioli and mounting immunity in tomato | en_US |
dc.type | Article | en_US |
Appears in Collections: | Institutional Publications |
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Jha G_2023_5.pdf Restricted Access | 3.67 MB | Adobe PDF | View/Open Request a copy |
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