Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1564
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dc.contributor.authorRengasamy, Balakrishnan-
dc.contributor.authorManna, Mrinalini-
dc.contributor.authorJonwal, Sarvesh-
dc.contributor.authorSathiyabama, Muthukrishnan-
dc.contributor.authorThajuddin, Nargis Begum-
dc.contributor.authorSinha, Alok Krishna-
dc.date.accessioned2024-01-16T09:49:57Z-
dc.date.available2024-01-16T09:49:57Z-
dc.date.issued2024-
dc.identifier.citationProtoplasma, 261(4): 641-654en_US
dc.identifier.issn1615-6102-
dc.identifier.issn0033-183X-
dc.identifier.otherhttps://doi.org/10.1007/s00709-023-01925-8-
dc.identifier.urihttps://link.springer.com/article/10.1007/s00709-023-01925-8-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/1564-
dc.descriptionAccepted date: 24 December 2023en_US
dc.description.abstractThe latest CRISPR-Cas9-mediated genome editing technology is expected to bring about revolution in rice yield and quality improvement, and thus validation of rice transformation protocols using CRISPR-Cas9-gRNA constructs is the need of the hour. Moreover, regeneration of more number of transgenic rice plants is prerequisite for developing genome-edited rice lines, as recalcitrant rice varieties were shown to have lower editing efciencies which necessities screening of large number of transgenic plants to fnd the suitable edits. In the present study, we have simplifed the Agrobacterium-mediated rice transformation protocol for both Indica and Japonica rice cultivars using CRISPR/Cas9 empty vector construct, and the protocols have been suitably optimized for getting large numbers of the regenerated plantlets within the shortest possible time. The Japonica transgenic lines were obtained within 65 days and for the Indica cultivars, it took about 76–78 days. We also obtained about 90% regeneration efciency for both Japonica and Indica cultivars. The transformation efciency was about 97% in the case of Japonica and 69–83% in the case of Indica rice cultivars. Furthermore, we screened the OsWRKY24 gene editing efciency by transforming rice cultivars with CRISPR/Cas9 construct harbouring sgRNA against OsWRKY24 gene and found about 90% editing efciency in Japonica rice cultivars, while 30% of the transformed Indica cultivars were found to be edited. This implicated the presence of a robust repair mechanism in the Indica rice cultivars.en_US
dc.description.sponsorshipThe work is supported by the core grant of the National Institute of Plant Genome Research from the Department of Biotechnology, Government of India. DST-SERB award was given to MM by the National Post-Doctoral Fellowship (SERB; File Number: PDF/2020/000511). The Department of Biotechnology, Government of India for the Senior Research fellowship was granted to SJ. The Sir J.C. Bose National Fellowship Award from SERB, Government of India was granted to AKS.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Nature Publishing AGen_US
dc.subjectRice transformationen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectIndica riceen_US
dc.subjectJaponica riceen_US
dc.subjectCRISPR-Cas9en_US
dc.subjectsgRNAen_US
dc.subjectRegeneration efciencyen_US
dc.subjectTransformation efciencyen_US
dc.subjectEditing efciencyen_US
dc.titleA simplified and improved protocol of rice transformation to cater wide range of rice cultivarsen_US
dc.typeArticleen_US
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