Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1578
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dc.contributor.authorManna, Mrinalini-
dc.contributor.authorRengasamy, Balakrishnan-
dc.contributor.authorSinha, Alok Krishna-
dc.date.accessioned2024-03-04T08:51:18Z-
dc.date.available2024-03-04T08:51:18Z-
dc.date.issued2024-
dc.identifier.citationPhytochemical Analysis, 35(5): 1052-1062en_US
dc.identifier.issn0958-0344-
dc.identifier.issn1099-1565-
dc.identifier.otherhttps://doi.org/10.1002/pca.3340-
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pca.3340-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/1578-
dc.descriptionAccepted date: 8 February 2024en_US
dc.description.abstractIntroduction Auxin estimation in plant tissues is a crucial component of auxin signaling studies. Despite the availability of various high-throughput auxin quantification methods like LC-MS, GC-MS, HPLC, biosensors, and DR5-gus/gfp-based assays, auxin quantification remains troublesome because these techniques are very expensive and technology intensive and they mostly involve elaborate sample preparation or require the development of transgenic plants. Objectives To find a solution to these problems, we made use of an old auxin detection system to quantify microbe derived auxins and modified it to effectively measure auxin levels in rice plants. Materials and methods Auxins from different tissues of rice plants, including root samples of seedlings exposed to IAA/TIBA or subjected to different abiotic stresses, were extracted in ethanol. The total auxin level was measured by the presently described colorimetric assay and counterchecked by other auxin estimation methods like LC-MS or gus staining of DR5-gus overexpressing lines. Results The presented colorimetric method could measure (1) the auxin levels in different tissues of rice plants, thus identifying the regions of higher auxin abundance, (2) the differential accumulation of auxins in rice roots when auxin or its transport inhibitor was supplied exogenously, and (3) the levels of auxin in roots of rice seedlings subjected to various abiotic stresses. The thus obtained auxin levels correlated well with the auxin levels determined by other methods like LC-MS or gus staining and the expression pattern of auxin biosynthesis pathway genes. Conclusions The auxin estimation method described here is simple, rapid, cost-effective, and sensitive and allows for the efficient detection of relative auxin abundances in plant tissues.en_US
dc.description.sponsorshipThe authors thank Dr. Jitender Giri, NIPGR, New Delhi, India for kindly providing the DR5-gus rice seeds. MM gratefully acknowledges SERB, DST, Government of India for awarding a National Post-Doctoral Fellowship (NPDF; File number: PDF/2020/000511). AKS acknowledges a Sir J.C. Bose National Fellowship Award from SERB, Government of India. The authors also acknowledge the service of the Metabolomics Facility and Central Instrumentation Facility of NIPGR, New Delhi, India. The authors are thankful to the DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. The authors gratefully acknowledge the contribution of Dr. Ruchi Agarrwal for improving the English language of the manuscript during revision.en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.subjectauxin estimationen_US
dc.subjectplant tissueen_US
dc.subjectrapid and robust methoden_US
dc.titleA rapid and robust colorimetric method for measuring relative abundance of auxins in plant tissuesen_US
dc.typeArticleen_US
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