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DC Field | Value | Language |
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dc.contributor.author | Sahu, Pranav Pankaj | - |
dc.contributor.author | Rai, Neeraj K. | - |
dc.contributor.author | Chakraborty, Supriya | - |
dc.contributor.author | Singh, Major | - |
dc.contributor.author | Chandrappa, Prasanna H. | - |
dc.contributor.author | Ramesh, Bandarupalli | - |
dc.contributor.author | Chattopadhyay, Debasis | - |
dc.contributor.author | Prasad, Manoj | - |
dc.date.accessioned | 2014-02-24T09:13:59Z | - |
dc.date.available | 2014-02-24T09:13:59Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Mol. Plant Pathology, 11(4): 531-544 | en_US |
dc.identifier.uri | http://hdl.handle.net/123456789/158 | - |
dc.description.abstract | Tomato leaf curl New Delhi virus (ToLCNDV) infection causes significant yield loss in tomato. The availability of a conventional tolerance source against this virus is limited in tomato. To understand the molecular mechanism of virus tolerance in tomato, the abundance of viral genomic replicative intermediate molecules and virus-directed short interfering RNAs (siRNAs) by the host plant in a naturally tolerant cultivar H-88-78-1 and a susceptible cultivar Punjab Chhuhara at different time points after agroinfection was studied. We report that less abundance of viral replicative intermediate in the tolerant cultivar may have a correlation with a relatively higher accumulation of virus-specific siRNAs. To study defence-related host gene expression in response to ToLCNDV infection, the suppression subtractive hybridization technique was used. A library was prepared from tolerant cultivar H-88-78-1 between ToLCNDV-inoculated and Agrobacterium mock-inoculated plants of this cultivar at 21 days post-inoculation (dpi). A total of 106 nonredundant transcripts was identified and classified into 12 different categories according to their putative functions. By reverse Northern analysis and quantitative real-time polymerase chain reaction (qRT-PCR), we identified the differential expression pattern of 106 transcripts, 34 of which were up-regulated (>2.5-fold induction). Of these, eight transcripts showed more than four fold induction. qRT-PCR analysis was carried out to obtain comparative expression profiling of these eight transcripts between Punjab Chhuhara and H-88-78-1 on ToLCNDV infection. The expression patterns of these transcripts showed a significant increase in differential expression in the tolerant cultivar, mostly at 14 and 21 dpi, in comparison with that in the susceptible cultivar, as analysed by qRT-PCR. The probable direct and indirect relationship of siRNA accumulation and up-regulated transcripts with the ToLCNDV tolerance mechanism is discussed. | en_US |
dc.description.sponsorship | We are grateful to the Department of Biotechnology, Government of India for providing financial support (Grant no. BT/PR/5274/AGR/16/464/2004). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.subject | New Delhi virus | en_US |
dc.subject | Tomato cultivar tolerant to Tomato leaf curl | en_US |
dc.subject | RNA accumulation | en_US |
dc.subject | Gene expression | en_US |
dc.title | Tomato cultivar tolerant to tomato leaf curl New Delhi Virus infection induces virus-specific siRNA accumulation and defense associated host gene expression | en_US |
dc.type | Article | en_US |
dc.date.AcceptedDate | May 2010 | en_US |
Appears in Collections: | Institutional Publications |
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