Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1591
Title: PROTEIN L-ISOASPARTYL METHYLTRANSFERASE protects enolase dysfunction by repairing isoaspartyl-induced damage and is positively implicated in agronomically important seed traits
Authors: Kamble, Nitin Uttam
Ghosh, Shraboni
Petla, Bhanu Prakash
Achary, Rakesh Kumar
Gautam, Shikha
Rao, Venkateswara
Salvi, Prafull
Hazra, Abhijit
Varshney, Vishal
Majee, Manoj
Keywords: Arabidopsis thaliana
PIMT
enolase
rice
seed size
seed vigor
Issue Date: 2024
Publisher: John Wiley & Sons
Citation: Plant Journal, 119(1): 413-431
Abstract: The protein-repairing enzyme (PRE) PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT) influences seed vigor by repairing isoaspartyl-mediated protein damage in seeds. However, PIMTs function in other seed traits, and the mechanisms by which PIMT affects such seed traits are still poorly understood. Herein, through molecular, biochemical, and genetic studies using overexpression and RNAi lines in Oryza sativa and Arabidopsis thaliana, we demonstrate that PIMT not only affects seed vigor but also affects seed size and weight by modulating enolase (ENO) activity. We have identified ENO2, a glycolytic enzyme, as a PIMT interacting protein through Y2H cDNA library screening, and this interaction was further validated by BiFC and co-immunoprecipitation assay. We show that mutation or suppression of ENO2 expression results in reduced seed vigor, seed size, and weight. We also proved that ENO2 undergoes isoAsp modification that affects its activity in both in vivo and in vitro conditions. Further, using MS/MS analyses, amino acid residues that undergo isoAsp modification in ENO2 were identified. We also demonstrate that PIMT repairs such isoAsp modification in ENO2 protein, protecting its vital cellular functions during seed maturation and storage, and plays a vital role in regulating seed size, weight, and seed vigor. Taken together, our study identified ENO2 as a novel substrate of PIMT, and both ENO2 and PIMT in turn implicate in agronomically important seed traits.
Description: Accepted date: 31 March 2024
URI: https://onlinelibrary.wiley.com/doi/10.1111/tpj.16771
http://223.31.159.10:8080/jspui/handle/123456789/1591
ISSN: 0960-7412
1365-313X
Appears in Collections:Institutional Publications

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