Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1594
Title: MicroRNA164e suppresses NAC100 transcription factor-mediated synthesis of seed storage proteins in chickpea
Authors: Chakraborty, Anirban
Singh, Baljinder
Pandey, Vimal
Parida, Swarup K.
Bhatia, Sabhyata
Keywords: NAC100
chickpea
microRNA164
seed protein content
seed storage proteins
transactivation
Issue Date: 2024
Publisher: John Wiley & Sons
Citation: New Phytologist, 242(6): 2652-2668
Abstract: Development of protein-enriched chickpea varieties necessitates an understanding of specific genes and key regulatory circuits that govern the synthesis of seed storage proteins (SSPs). Here, we demonstrated the novel involvement of Ca-miR164e-CaNAC100 in regulating SSP synthesis in chickpea. Ca-miRNA164e was significantly decreased during seed maturation, especially in high-protein accessions. The miRNA was found to directly target the transactivation conferring C-terminal region of a nuclear-localized transcription factor, CaNAC100 as revealed using RNA ligase-mediated-rapid amplification of cDNA ends and target mimic assays. The functional role of CaNAC100 was demonstrated through seed-specific overexpression (NACOE) resulting in significantly augmented seed protein content (SPC) consequential to increased SSP transcription. Further, NACOE lines displayed conspicuously enhanced seed weight but reduced numbers and yield. Conversely, a downregulation of CaNAC100 and SSP transcripts was evident in seed-specific overexpression lines of Ca-miR164e that culminated in significantly lowered SPC. CaNAC100 was additionally demonstrated to transactivate the SSP-encoding genes by directly binding to their promoters as demonstrated using electrophoretic mobility shift and dual-luciferase reporter assays. Taken together, our study for the first time established a distinct role of CaNAC100 in positively influencing SSP synthesis and its critical regulation by CamiR164e, thereby serving as an understanding that can be utilized for developing SPC-rich chickpea varieties.
Description: Accepted date: 27 March 2024
URI: https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19770
http://223.31.159.10:8080/jspui/handle/123456789/1594
ISSN: 0028-646X
1469-8137
Appears in Collections:Institutional Publications

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