Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/1618
Title: Measurement of reactive oxygen species and nitric oxide from tomato plants in response to abiotic and biotic stresses
Authors: Samant, Sanjib Bal
Manbir
Rekha
Swain, Jagannath
Singh, Pooja
Kumari, Aprajita
Gupta, Kapuganti Jagadis
Keywords: Botrytis cenerea
Nitric oxide
Nitrosative stress
Osmotic stress
Reactive oxygen species
Issue Date: 2024
Publisher: Springer Nature Publishing AG
Citation: Methods in Molecular Biology, 2832: 183-203
Abstract: Nitric oxide (NO) is a free radical molecule that has been known to influence several cellular processes such as plant growth, development, and stress responses. NO together with reactive oxygen species (ROS) play a role in signaling process. Due to extremely low half-life of these radicals in cellular environment, it is often difficult to precisely monitor them. Each method has some advantages and disadvantages; hence, it is important to measure using multiple methods. To interpret the role of each signaling molecule in numerous biological processes, sensitive and focused methods must be used. In addition to this complexity, these Reactive Oxygen Species (ROS) and NO react with each other leads to nitro-oxidative stress in plants. Using tomato as a model system here, we demonstrate stepwise protocols for measurement of NO by chemiluminescence, DAF fluorescence, nitrosative stress by western blot, and ROS measurement by NBT and DAB under stress conditions such as osmotic stress and Botrytis infection. While describing methods, we also emphasized on benefits, drawbacks, and broader applications of these methods.
Description: Accepted date: 14 June 2024
URI: https://link.springer.com/protocol/10.1007/978-1-0716-3973-3_13
http://223.31.159.10:8080/jspui/handle/123456789/1618
ISBN: 978-1-0716-3972-6
978-1-0716-3973-3
Appears in Collections:Institutional Publications

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