Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/218
Full metadata record
DC FieldValueLanguage
dc.contributor.authorYadav, Rajiv Kumar-
dc.contributor.authorChattopadhyay, Debasis-
dc.date.accessioned2014-05-01T06:41:22Z-
dc.date.available2014-05-01T06:41:22Z-
dc.date.issued2011-
dc.identifier.citationMol. Plant Microbe Interactions, 24(10): 1189-1197en_US
dc.identifier.urihttp://hdl.handle.net/123456789/218-
dc.description.abstractRNA silencing mediated by short-interfering RNA (siRNA) is used by plants as a defense against viruses. In the case of geminiviruses, viral DNA is targeted at the transcriptional level, while virus-derived transcripts are targeted by posttranscriptional silencing. Mungbean yellow mosaic India virus (MYMIV), a bipartite geminivirus, causes yellow mosaic disease in soybean (Glycine max). A soybean variety resistant to this disease has been identified (line PK416). To understand the molecular mechanism underlying this resistance, distribution of MYMIV-derived siRNAs along the viral genome was compared in resistant and susceptible plants, using samples obtained in the first few days following inoculation. We observed that, in the resistant soybean variety, most of the virus-derived siRNAs were complementary to the intergenic region (IR), while in the susceptible variety (line JS335), a majority of the siRNAs corresponded to coding regions of the viral genome. Most of the IR-specific siRNA molecules produced in the resistant plants were 24 nt in size. Bisulfite sequencing showed that, in the resistant plants, a higher level of methylation occurred in the IR of viral DNA.en_US
dc.description.sponsorshipThis work was supported by funding from National Institute of Plant Genome Research, New Delhi (NIPGR).en_US
dc.language.isoenen_US
dc.publisherAmerican Phytopathological Societyen_US
dc.subjectGeminivirusen_US
dc.subjectRNA Accumulationen_US
dc.subjectDNA Methylationen_US
dc.subjectViralen_US
dc.titleEnhanced viral intergenic region specific siRNA accumulation and DNA methylation correlates with resistance against a geminivirusen_US
dc.typeArticleen_US
dc.date.AcceptedDate13 June 2011en_US
Appears in Collections:Institutional Publications

Files in This Item:
File Description SizeFormat 
Chattopadhyay D_2011_1.pdf
  Restricted Access
717.49 kBAdobe PDFView/Open Request a copy


Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.