Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/262
Title: The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet
Authors: Mishra, Awdhesh Kumar
Puranik, Swati
Bahadur, Ranjit Prasad
Prasad, Manoj
Keywords: Abiotic stress
Protoplast co-transfection
Dehydration-responsive element (DRE)
Protein–protein docking
Setaria italica
WD40 protein
Electrophoretic mobility shift assay (EMSA)
Issue Date: 2012
Publisher: Elsevier
Citation: Genomics, 100: 252-263
Abstract: A differentially expressed transcript, encoding a putative WD protein (Setaria italica WD40; SiWD40), was identified in foxtail millet. Tertiary structure modeling revealed that its C-terminus possesses eight blade β-propeller architecture. Its N-terminal has three α-helices and two 310-helices and was highly induced by different abiotic stresses. The SiWD40:GFP fusion protein was nuclear localized. Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (DRE). A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter. Thus, for the first time, we report that DREs probably regulate expression of SiWD40 during envi- ronmental stress. Molecular docking analysis revealed that the circumference of the β‐propeller structure was involved in an interaction with a SiCullin4 protein, supporting the adaptability of SiWD40 to act as a scaf- fold. Our study thus provides a vital clue for near future research on the stress-regulation of WD proteins.
URI: http://172.16.0.77:8080/jspui/handle/123456789/262
ISSN: 0888-7543
Appears in Collections:Institutional Publications

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