Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/376
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dc.contributor.authorSubba, Pratigya-
dc.contributor.authorKumar, Rajiv-
dc.contributor.authorGayali, Saurabh-
dc.contributor.authorShekhar, Shubhendu-
dc.contributor.authorParveen, Shaista-
dc.contributor.authorPandey, Aarti-
dc.contributor.authorDatta, Asis-
dc.contributor.authorChakraborty, Subhra-
dc.contributor.authorChakraborty, Niranjan-
dc.date.accessioned2015-11-24T08:42:03Z-
dc.date.available2015-11-24T08:42:03Z-
dc.date.issued2013-
dc.identifier.citationProteomics, 13(12-13): 1973-1992en_US
dc.identifier.issn1615-9861-
dc.identifier.urihttp://172.16.0.77:8080/jspui/handle/123456789/376-
dc.descriptionAccepted date: March 19, 2013en_US
dc.description.abstractWater deficit or dehydration hampers plant growth and development, and shrinks harvest size of major crop species worldwide. Therefore, a better understanding of dehydration response is the key to decipher the regulatory mechanism of better adaptation. In recent years, nuclear proteomics has become an attractive area of research, particularly to study the role of nucleus in stress response. In this study, a proteome of dehydration-sensitive chickpea cultivar (ICCV-2) was generated from nuclei-enriched fractions. The LC-MS/MS analysis led to the identification of 75 differentially expressed proteins presumably associated with different metabolic and regulatory pathways. Nuclear localisation of three candidate proteins was validated by transient expression assay. The ICCV-2 proteome was then compared with that of JG-62, a tolerant cultivar. The differential proteomics and in silico analysis revealed cultivar-specific differential expression of many proteins involved in various cellular functions. The differential tolerance could be attributed to altered expression of many structural proteins and the proteins involved in stress adaptation, notably the ROS catabolising enzymes. Further, a comprehensive comparison on the abiotic stress-responsive nuclear proteome was performed using the datasets published thus far. These findings might expedite the functional determination of the dehydration-responsive proteins and their prioritisation as potential molecular targets for better adaptation.en_US
dc.description.sponsorshipThis work was supported by grants (BT/PR/10677/ PBD/16/795) from the Department of Biotechnology (DBT), Government of India. We thank the Council of Scientific and Industrial Research (CSIR), Government of India and the DBT for providing predoctoral fellowship to PS, RK, SG and SP. We thank Dr. Suchismita Dass for critical reading of the manuscript and Mr. Jasbeer Singh for illustrations and graphical representation in the manuscript.en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sons Ltden_US
dc.subjectAdaptive strategiesen_US
dc.subjectComparative proteomicsen_US
dc.subjectDehydrationen_US
dc.subjectNuclei-enriched fractionen_US
dc.subjectPulse legumeen_US
dc.subjectROS catabolising enzymesen_US
dc.titleCharacterization of the nuclear proteome of a dehydration-sensitive cultivar of chickpea and comparative proteomic analysis with a tolerant cultivaren_US
dc.typeArticleen_US
dc.identifier.officialurlhttp://onlinelibrary.wiley.com/doi/10.1002/pmic.201200380/abstracten_US
dc.identifier.doi10.1002/pmic.201200380en_US
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