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http://223.31.159.10:8080/jspui/handle/123456789/451
Title: | Natural allelic diversity, genetic structure and linkage disequilibrium pattern in wild chickpea |
Authors: | Saxena, Maneesha S. Bajaj, Deepak Kujur, Alice Das, Shouvik Badoni, Saurabh Kumar, Vinod Singh, Mohar Bansal, Kailash C. Tyagi, Akhilesh K. Parida, Swarup K. |
Keywords: | Wild Chickpea Chickpea natural allelic diversity |
Issue Date: | 2014 |
Publisher: | PLOS |
Citation: | PLoS One, 9(9): e107484 |
Abstract: | Characterization of natural allelic diversity and understanding the genetic structure and linkage disequilibrium (LD) pattern in wild germplasm accessions by large-scale genotyping of informative microsatellite and single nucleotide polymorphism (SNP) markers is requisite to facilitate chickpea genetic improvement. Large-scale validation and high-throughput genotyping of genome-wide physically mapped 478 genic and genomic microsatellite markers and 380 transcription factor gene-derived SNP markers using gel-based assay, fluorescent dye-labelled automated fragment analyser and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass array have been performed. Outcome revealed their high genotyping success rate (97.5%) and existence of a high level of natural allelic diversity among 94 wild and cultivated Cicer accessions. High intra- and inter-specific polymorphic potential and wider molecular diversity (11-94%) along with a broader genetic base (13-78%) specifically in the functional genic regions of wild accessions was assayed by mapped markers. It suggested their utility in monitoring introgression and transferring target trait-specific genomic (gene) regions from wild to cultivated gene pool for the genetic enhancement. Distinct species/gene pool-wise differentiation, admixed domestication pattern, and differential genome-wide recombination and LD estimates/decay observed in a six structured population of wild and cultivated accessions using mapped markers further signifies their usefulness in chickpea genetics, genomics and breeding. |
Description: | Accepted date: August 11, 2014 |
URI: | http://172.16.0.77:8080/jspui/handle/123456789/451 |
ISSN: | 1932-6203 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Parida SK_2014_3.pdf | 1.09 MB | Adobe PDF | ![]() View/Open |
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