Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/474
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dc.contributor.authorVerma, Priyanka-
dc.contributor.authorSharma, Tilak R.-
dc.contributor.authorSrivastava, Prem S.-
dc.contributor.authorAbdin, M. Z.-
dc.contributor.authorBhatia, Sabhyata-
dc.date.accessioned2015-12-29T11:34:48Z-
dc.date.available2015-12-29T11:34:48Z-
dc.date.issued2014-
dc.identifier.citationMol. Biol. Rep., 41(9): 5607-5625en_US
dc.identifier.issn1573-4978-
dc.identifier.urihttp://172.16.0.77:8080/jspui/handle/123456789/474-
dc.descriptionAccepted date: 21 May 2014en_US
dc.description.abstractLentil (Lens culinaris Medik.) is an economically important grain legume, yet the genetic and genomic resources remain largely uncharacterized and unexploited in this crop. Microsatellites have become markers of choice for crop improvement applications. Hence, simple sequence repeat (SSR) markers were developed for lentil through the construction of genomic library enriched for GA/CT motifs. As a result 122 functional SSR primer pairs were developed from 151 microsatellite loci and validated in L. culinaris cv. Precoz. Thirty three SSR markers were utilized for the analysis of genetic relationships between cultivated and wild species of Lens and related legumes. A total of 123 alleles were amplified at 33 loci ranging from 2-5 alleles with an average of 3.73 alleles per locus. Polymorphic information content (PIC) for all the loci ranged from 0.13 to 0.99 with an average of 0.66 per locus. Varied levels of cross genera transferability were obtained ranging from 69.70 % across Pisum sativum to 12.12 % across Vigna radiata. The UPGMA based dendrogram was able to establish the uniqueness of each genotype and grouped them into two major clusters clearly resolving the genetic relationships within lentil and related species. The new set of SSR markers reported here were efficient and highly polymorphic and would add to the existing repertoire of lentil SSR markers to be utilized in molecular breeding. Moreover, the improved knowledge about intra- and inter-specific genetic relationships would facilitate germplasm utilization for lentil improvement.en_US
dc.description.sponsorshipWe are grateful to Dr. T.R. Sharma (CSK, Himachal Pradesh University, Palampur) for providing the accessions of lentil. We are grateful to the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) India, National Bureau for Plant Genetic Resources (NBPGR) India, Maharana Pratap Agriculture University (MPAU) India, and Australian Medicago Genetic Resource Centre, SARDI, Australia for providing us the seed material of different accessions of legumes. This research work was supported by the National Institute of Plant Genome Research (NIPGR), New Delhi, India. Financial assistance was supported by The Department of Biotechnology (DBT), Government of India, under the Grant No. BT/01/08/NRC-PBI/01.en_US
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.subjectMicrosatellitesen_US
dc.subjectSSRsen_US
dc.subjectLentilen_US
dc.subjectEnriched libraryen_US
dc.subjectGenetic diversityen_US
dc.subjectCross-genera transferabilityen_US
dc.titleExploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markersen_US
dc.typeArticleen_US
dc.identifier.officialurlhttp://link.springer.com/article/10.1007%2Fs11033-014-3431-zen_US
dc.identifier.doi10.1007/s11033-014-3431-zen_US
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