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DC Field | Value | Language |
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dc.contributor.author | Irfan, Mohammad | - |
dc.contributor.author | Ghosh, Sumit | - |
dc.contributor.author | Meli, Vijaykumar S. | - |
dc.contributor.author | Kumar, Anil | - |
dc.contributor.author | Kumar, Vinay | - |
dc.contributor.author | Chakraborty, Niranjan | - |
dc.contributor.author | Chakraborty, Subhra | - |
dc.contributor.author | Datta, Asis | - |
dc.date.accessioned | 2016-02-09T05:32:18Z | - |
dc.date.available | 2016-02-09T05:32:18Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Front. Plant Sc., 7: 10 | en_US |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://172.16.0.77:8080/jspui/handle/123456789/619 | - |
dc.description | Accepted date: 07 January 2016 | en_US |
dc.description.abstract | α-Mannosidase (α-Man), a fruit ripening-specific N-glycan processing enzyme, is involved in ripening-associated fruit softening process. However, the regulation of fruit-ripening specific expression of α-Man is not well understood. We have identified and functionally characterized the promoter of tomato (Solanum lycopersicum) α-Man to provide molecular insights into its transcriptional regulation during fruit ripening. Fruit ripening-specific activation of the α-Man promoter was revealed by analysing promoter driven expression of beta-glucuronidase (GUS) reporter in transgenic tomato. We found that RIPENING INHIBITOR (RIN), a MADS box family transcription factor acts as positive transcriptional regulator of α-Man during fruit ripening. RIN directly bound to the α-Man promoter sequence and promoter activation/α-Man expression was compromised in rin mutant fruit. Deletion analysis revealed that a promoter fragment (567 bp upstream of translational start site) that contained three CArG boxes (binding sites for RIN) was sufficient to drive GUS expression in fruits. In addition, α-Man expression was down-regulated in fruits of Nr mutant which is impaired in ethylene perception and promoter activation/α-Man expression was induced in wild type following treatment with a precursor of ethylene biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC). Although, α-Man expression was induced in rin mutant after ACC treatment, the transcript level was less as compared to ACC-treated wild type. Taken together, these results suggest RIN-mediated direct transcriptional regulation of α-Man during fruit ripening and ethylene may acts in RIN-dependent and -independent ways to regulate α-Man expression. | en_US |
dc.description.sponsorship | The present study was financially supported by a research grant (BT/01/CEIB/12/II/01) from the Department of Biotechnology and core research grant from National Institute of Plant Genome Research. MI thanks University Grant Commission for pre-doctoral fellowship. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Frontiers Media S.A. | en_US |
dc.subject | fruit ripening | en_US |
dc.subject | transcriptional regulation | en_US |
dc.subject | fruit ripening-specific promoter | en_US |
dc.subject | α-Man | en_US |
dc.subject | RIN | en_US |
dc.title | Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | http://journal.frontiersin.org/article/10.3389/fpls.2016.00010/abstract | en_US |
dc.identifier.doi | 10.3389/fpls.2016.00010 | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Datta A_2016_2.pdf | 2.1 MB | Adobe PDF | ![]() View/Open |
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