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DC Field | Value | Language |
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dc.contributor.author | Srivastava, Rishi | - |
dc.contributor.author | Bajaj, Deepak | - |
dc.contributor.author | Malik, Ayushi | - |
dc.contributor.author | Singh, Mohar | - |
dc.contributor.author | Parida, Swarup K. | - |
dc.date.accessioned | 2016-10-04T10:27:19Z | - |
dc.date.available | 2016-10-04T10:27:19Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Scientific Reports, 6: 33616 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://59.163.192.83:8080/jspui/handle/123456789/686 | - |
dc.description | Accepted date: 26 August 2016 | en_US |
dc.description.abstract | The RNA-sequencing followed by de-novo transcriptome assembly identified 11621 genes differentially xpressed in roots vs. shoots of a wild perennial Cicer microphyllum. Comparative analysis of transcriptomes between microphyllum and cultivated desi cv. ICC4958 detected 12772 including 3242 root- and 1639 shoot-specific microphyllum genes with 85% expression validation success rate. Transcriptional reprogramming of microphyllum root-specific genes implicates their possible role in regulating differential natural adaptive characteristics between wild and cultivated chickpea. The transcript-derived 5698 including 282 in-silico polymorphic SSR and 127038 SNP markers annotated at a genome-wide scale exhibited high amplification and polymorphic potential among cultivated (desi and kabuli) and wild accessions suggesting their utility in chickpea genomics-assisted breeding applications. The functional significance of markers was assessed based on their localization in non-synonymous coding and regulatory regions of microphyllum root-specific genes differentially expressed predominantly in ICC 4958 roots under drought stress. A high-density 490 genic SSR- and SNP markers-anchored genetic linkage map identified six major QTLs regulating drought tolerance-related traits, yield per plant and harvest-index in chickpea. The integration of high-resolution QTL mapping with comparative transcriptome profiling delineated five microphyllum root-specific genes with non-synonymous and regulatory SNPs governing drought-responsive yield traits. Multiple potential key regulators and functionally relevant molecular tags delineated can drive translational research and drought tolerance-mediated chickpea genetic enhancement. | en_US |
dc.description.sponsorship | The authors gratefully acknowledge the financial support for this study provided by a research grant from the Department of Biotechnology (DBT), Government of India (102/IFD/SAN/2161/2013-14). The authors also acknowledge the financial support provided by the Department of Agriculture and Co-operation (DAC), Government of India under the National Food Security Mission. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Natural variation in plants | en_US |
dc.subject | Cicer microphyllum | en_US |
dc.title | Transcriptome landscape of perennial wild Cicer microphyllum uncovers functionally relevant molecular tags regulating agronomic traits in chickpea | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | http://www.nature.com/articles/srep33616?WT.feed_name=subjects_genetics | en_US |
dc.identifier.doi | 10.1038/srep33616 | en_US |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Parida SK_2016_8.pdf | 2.46 MB | Adobe PDF | View/Open |
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