Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/703
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dc.contributor.authorJaijyan, Dabbu Kumar-
dc.contributor.authorVerma, Praveen K.-
dc.contributor.authorSingh, Agam Prasad-
dc.date.accessioned2016-12-26T10:35:59Z-
dc.date.available2016-12-26T10:35:59Z-
dc.date.issued2016-
dc.identifier.citationScientific Reports, 6: 39285en_US
dc.identifier.issn2045-2322-
dc.identifier.urihttp://59.163.192.83:8080/jspui/handle/123456789/703-
dc.descriptionAccepted date: 22 November 2016en_US
dc.description.abstractProtein phosphorylation is the most important post-translational event in the regulation of various essential signaling pathways in a cell. Here, we show the functional characterization of a FIKK family protein kinase of the rodent malaria parasite (PbMLFK), which is expressed only in mosquito and liver stages and contains two functional C-terminal PEXEL motifs. We demonstrate that this protein plays a role in mosquito and liver stages of parasite growth. The oocysts of PbMLFK-deficient parasites produced 4-fold fewer sporozoites. In the liver of infected mice, PbMLFK-deficient parasites grew 100-fold less than did wild type parasites. We also show that the C-terminal domain of this protein has a functional serine-threonine kinase and that its activity was inhibited by a known PKA inhibitor. Transcriptome analysis of infected host cells suggests that in absence of this protein expression of the 288 host mRNAs are perturbed which are primarily associated with the immune system, cell cycle and metabolism.en_US
dc.description.sponsorshipWe thank Pradeep Kumar Tiwari and Vivek Kumar Pandey for their technical assistance in mosquito breeding and animal handling. We thank Dr. Arnab Mukhopadhyay and Dr. Awadhesh Pandit for their help in collecting the NGS data at the NII core facility. We also thank Vikash Kumar at the NII central FACS facility for cell sorting.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.subjectParasite developmenten_US
dc.subjectTranscriptomicsen_US
dc.subjectmalariaen_US
dc.subjectFIKK kinaseen_US
dc.titleA novel FIKK kinase regulates the development of mosquito and liver stages of the malariaen_US
dc.typeArticleen_US
dc.identifier.officialurlhttp://www.nature.com/articles/srep39285?WT.feed_name=subjects_molecular-biologyen_US
dc.identifier.doi10.1038/srep39285en_US
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