Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/756
Title: | Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana |
Authors: | Singh, Archita Roy, Shradha Singh, Sharmila Das, Shabari Sarkar Gautam, Vibhav Yadav, Sandeep Kumar, Ashutosh Singh, Alka Samantha, Sukanya Sarkar, Ananda K. |
Keywords: | Plant physiology Plant molecular biology Arabidopsis thaliana KANADI genes Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) Phytohormonal |
Issue Date: | 2017 |
Publisher: | Nature Publishing Group |
Citation: | Scientific Reports, 7(1): 3408 |
Abstract: | Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk. |
Description: | Accepted date: 19 April 2017 |
URI: | http://59.163.192.83:8080/jspui/handle/123456789/756 |
ISSN: | 2045-2322 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sarkar AK_2017_3.pdf | 2.4 MB | Adobe PDF | View/Open |
Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.