Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/756
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Singh, Archita | - |
dc.contributor.author | Roy, Shradha | - |
dc.contributor.author | Singh, Sharmila | - |
dc.contributor.author | Das, Shabari Sarkar | - |
dc.contributor.author | Gautam, Vibhav | - |
dc.contributor.author | Yadav, Sandeep | - |
dc.contributor.author | Kumar, Ashutosh | - |
dc.contributor.author | Singh, Alka | - |
dc.contributor.author | Samantha, Sukanya | - |
dc.contributor.author | Sarkar, Ananda K. | - |
dc.date.accessioned | 2017-06-16T08:50:30Z | - |
dc.date.available | 2017-06-16T08:50:30Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Scientific Reports, 7(1): 3408 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://59.163.192.83:8080/jspui/handle/123456789/756 | - |
dc.description | Accepted date: 19 April 2017 | en_US |
dc.description.abstract | Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk. | en_US |
dc.description.sponsorship | We thank Department of Biotechnology (DBT, Govt. of India) for Ramalingaswami fellowship (BT/HRD/35/02/06/2008) grant to A.K.S. and UGC for fellowship to S.Y. Council of Scientific and Industrial Research (CSIR) provided fellowship to A.S., S.S., and V.G., and NIPGR provided internal funding, fellowship to A.K. is provided by SERB-NPDF (PDF/2015/000232) and is duly acknowledged. Fellowship to Al.S. is provided by DBT-RA and is duly acknowledged. Su.S. acknowledges NIPGR for internal grants and fellowship. SSD acknowledges Women Scientist-A (Wos-A) fellowship from Department of Science and Technology (DST), India WOS-A/LS- 1276/2014. We thank Dr. J.W. Reed, Dr. A.K. Nandi and ABRC for providing us with shy2-2, npr1-2 and other seed materials, respectively. We thank Suvakanta Barik for help with initial studies on cis-elements of MIR166s and HD-ZIP IIIs. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.subject | Plant physiology | en_US |
dc.subject | Plant molecular biology | en_US |
dc.subject | Arabidopsis thaliana | en_US |
dc.subject | KANADI genes | en_US |
dc.subject | Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) | en_US |
dc.subject | Phytohormonal | en_US |
dc.title | Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://www.nature.com/articles/s41598-017-03632-w | en_US |
dc.identifier.doi | 10.1038/s41598-017-03632-w | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sarkar AK_2017_3.pdf | 2.4 MB | Adobe PDF | View/Open |
Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.