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http://223.31.159.10:8080/jspui/handle/123456789/762
Title: | Global transcriptome and co-expression network analyses reveal cultivar-specific molecular signatures associated with seed development and seed size/weight determination in chickpea |
Authors: | Garg, Rohini Singh, Vikash K. Rajkumar, Mohan Singh Kumar, Vinay Jain, Mukesh |
Keywords: | Cicer arietinum GSE79719 GSE79720 co-expression network gene expression seed development seed size/weight transcriptional modules transcriptome dynamics |
Issue Date: | 2017 |
Publisher: | John Wiley & Sons |
Citation: | Plant Journal, 91(6):1088-1107 |
Abstract: | Seed development is an intricate process regulated via a complex transcriptional regulatory network. To understand the molecular mechanisms governing seed development and seed size/weight in chickpea, we performed a comprehensive analysis of transcriptome dynamics during seed development in two cultivars with contrasting seed size/weight (small seeded, Himchana 1 and large-seeded, JGK 3). Our analysis identified stage-specific expression for a significant proportion (>13%) of the genes. About one-fourth of total genes exhibited significant differential expression in JGK 3 as compared to Himchana 1. We found that different seed development stages can be delineated by modules of co-expressed genes. A comparative analysis revealed differential developmental stage specificity of some modules between the two cultivars. Furthermore, we constructed transcriptional regulatory networks and identified key components determining seed size/weight. The results suggested that extended period of cell division during embryogenesis and higher level of endoreduplication along with more accumulation of storage compounds during maturation determine large seed size/weight. Further, we identified quantitative trait loci-associated candidate genes harboring single nucleotide polymorphisms in the promoter sequences that differentiate small- and large-seeded chickpea cultivars. The results provide a valuable resource to dissect the role of candidate genes governing seed development and seed size/weight in chickpea. |
Description: | Accepted date: 22 June 2017 |
URI: | http://59.163.192.83:8080/jspui/handle/123456789/762 |
ISSN: | 1365-313X |
Appears in Collections: | Institutional Publications |
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Jain M_2017_2.pdf Restricted Access | 3.04 MB | Adobe PDF | View/Open Request a copy |
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