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DC Field | Value | Language |
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dc.contributor.author | Balyan, Sonia | - |
dc.contributor.author | Kumar, Mukesh | - |
dc.contributor.author | Mutum, Roseeta Devi | - |
dc.contributor.author | Raghuvanshi, Utkarsh | - |
dc.contributor.author | Agarwal, Priyanka | - |
dc.contributor.author | Mathur, Saloni | - |
dc.contributor.author | Raghuvanshi, Saurabh | - |
dc.date.accessioned | 2017-11-16T10:14:35Z | - |
dc.date.available | 2017-11-16T10:14:35Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Scientific Reports, 7(1): 15446 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/798 | - |
dc.description | Accepted date: 23 October 2017 | en_US |
dc.description.abstract | Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs "Cultivar-specific drought responsive" (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought. Interestingly, CSDR-miRNAs target several copper-protein coding transcripts like plantacyanins, laccases and Copper/Zinc superoxide dismutases (Cu/Zn SODs) and are themselves found to be similarly induced under simulated copper-starvation in both N22 and PB1. Transcription factor OsSPL9, implicated in Cu-homeostasis also interacted with osa-miR408-3p and osa-miR528-5p promoters. Further, N22 flag leaves showed lower SOD activity, accumulated ROS and had a higher stomata closure. Interestingly, compared to PB1, internal Cu levels significantly decreased in the N22 flag-leaves, during drought. Thus, the study identifies the unique drought mediated dynamism and interplay of Cu and ROS homeostasis, in the flag leaves of drought tolerant rice, wherein CSDR-miRNAs play a pivotal role. | en_US |
dc.description.sponsorship | Tis work was funded by the Department of Biotechnology (DBT), Government of India (Grant no. BT/PR10071/ AGR/36/31/2007 and BT/PR628/AGR/36/674/2011) and the ‘Scheme to strengthen R&D Doctoral Research program’ of Univ. of Delhi, Delhi, India. S.C.B. sincerely thanks the University of Delhi for the UTA fellowship for doctoral research. R.D.M. acknowledges University Grant Commission and U.R acknowledge Department of Biotechnology (DBT) for fellowships. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.subject | Drought | en_US |
dc.subject | Plant molecular biology | en_US |
dc.subject | Nagina 22 | en_US |
dc.subject | drought tolerant | en_US |
dc.subject | rice | en_US |
dc.subject | miRNA-mediated | en_US |
dc.title | Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22 | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://www.nature.com/articles/s41598-017-15450-1 | en_US |
dc.identifier.doi | 10.1038/s41598-017-15450-1 | en_US |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Mathur S_2017_1.pdf | 5.82 MB | Adobe PDF | View/Open |
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