Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/801
Title: Quantitative extracellular matrix proteomics suggests cell wall reprogramming in host-specific immunity during vascular wilt caused by Fusarium oxysporum in chickpea
Authors: Elagamey, Eman
Narula, Kanika
Sinha, Arunima
Ghosh, Sudip
Abdellatef, Magdi A.E.
Chakraborty, Niranjan
Chakraborty, Subhra
Keywords: Chickpea
Comparative proteomics and protein network
Extracellular matrix
Fusarium oxysporum
Innate immunity
Vascular wilt
Issue Date: 2017
Publisher: John Wiley & Sons
Citation: Proteomics, 17(23-24): 1600374
Abstract: Extracellular matrix (ECM) is the unique organelle that perceives stress signals and reprograms molecular events of host cell during patho-stress. However, our understanding of how ECM dictates plant immunity is largely unknown. Vascular wilt caused by the soil borne filamentous fungus Fusarium oxysporum is a major impediment for global crop productivity. To elucidate the role of ECM proteins and molecular mechanism associated with cell wall mediated immunity, the temporal changes of ECM proteome was studied in vascular wilt resistant chickpea cultivar upon F. oxysporum infection. The 2-DE protein profiling coupled with mass spectrometric analysis identified 166 immune responsive proteins (IRPs) involved in variety of functions. Our data suggest that wall remodeling; protein translocation, stabilization and chitin triggered immunity; extracellular ATP signaling are major players in early, middle and later phases of ECM signaling during fungal attack. Furthermore, we interrogated the proteome data using network analysis that identified modules enriched in known and novel immunity-related prognostic proteins centered around nascent aminopolypeptide complex (NAC), amine oxidase, thioredoxin and chaperonin. This study for the first time provides an insight into the complex network operating in the ECM and impinges on the surveillance mechanism of innate immunity during patho-stress in crop plant.
Description: Accepted date: 2 November 2017
URI: http://223.31.159.10:8080/jspui/handle/123456789/801
ISSN: 1615-9861
Appears in Collections:Institutional Publications

Files in This Item:
File Description SizeFormat 
Chakraborty S_2017_4.pdf
  Restricted Access
6.3 MBAdobe PDFView/Open Request a copy


Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.