Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/802
Title: Interplay of neuronal and non-neuronal genes regulates intestinal DAF-16-mediated immune response during Fusarium infection of Caenorhabditis elegans
Authors: Nag, Papri
Aggarwal, Pooja Rani
Ghosh, Sudip
Narula, Kanika
Tayal, Rajul
Maheshwari, Nidhi
Chakraborty, Niranjan
Chakraborty, Subhra
Keywords: Fungal infection
Transcriptomics
Fusarium
Caenorhabditis elegans
neuronal and non-neuronal genes
DAF-16-mediated immune
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Cell Death Discovery, 3: 17073
Abstract: Although precisely controlled innate immune response is governed by conserved cellular events in phylogenetically diverse hosts, the underlying molecular mechanisms by which this process is regulated against a multi-host pathogen remain unknown. Fusarium oxysporum is a model multi-host pathogen, known to be associated with neuronal stress in humans and vascular wilt in plants. The interaction between innate immune and neuronal pathways is the basis of many diverse biological responses. How these processes are coordinated in response to fungal disease is not well understood. Here, we show that F. oxysporum f. sp. ciceri causes neuronal stress and intestinal disintegration, ultimately leading to the death of Caenorhabditis elegans. To explore the regulatory framework of Fusarium-associated disease, we analysed the gene expression during infection, integrated temporal gene expression, and network analysis with genetic inactivation data in Caenorhabditis elegans. We identified 1024 genes showing significant changes in expression (corrected P-values <0.05) in response to Fusarium infection. Co-expression network analysis of our data identified prognostic genes related to disease progression. These genes were dynamically expressed in various neuronal and non-neuronal tissues exhibiting diverse biological functions, including cellular homeostasis, organ patterning, stress response, and lipid metabolism. The RNA-seq analysis further identified shared and unique signalling pathways regulated by DAF-16/FOXO and SIR-2.1 linking neuronal stress, which facilitates negative regulation of intestinal innate immunity. Genetic analysis revealed that GCY-5 in ASE functions upstream of DAF-16, whereas ASI-specific SRD-1 regulates behavioural immunity. Overall, our results indicate that a ubiquitous response occurs during Fusarium infection mediated by highly conserved regulatory components and pathways, which can be exploited further for the identification of disease-responsive genes conserved among animals and plants. Finally, this study provided a novel insight into cross-species immune signalling and may facilitate the discovery of cellular therapeutic targets for Fusarium-associated disease.
Description: Accepted date: 13 September 2017
URI: http://223.31.159.10:8080/jspui/handle/123456789/802
ISSN: 2058-7716
Appears in Collections:Institutional Publications

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