Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/802
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNag, Papri-
dc.contributor.authorAggarwal, Pooja Rani-
dc.contributor.authorGhosh, Sudip-
dc.contributor.authorNarula, Kanika-
dc.contributor.authorTayal, Rajul-
dc.contributor.authorMaheshwari, Nidhi-
dc.contributor.authorChakraborty, Niranjan-
dc.contributor.authorChakraborty, Subhra-
dc.date.accessioned2017-11-22T06:01:52Z-
dc.date.available2017-11-22T06:01:52Z-
dc.date.issued2017-
dc.identifier.citationCell Death Discovery, 3: 17073en_US
dc.identifier.issn2058-7716-
dc.identifier.urihttp://223.31.159.10:8080/jspui/handle/123456789/802-
dc.descriptionAccepted date: 13 September 2017en_US
dc.description.abstractAlthough precisely controlled innate immune response is governed by conserved cellular events in phylogenetically diverse hosts, the underlying molecular mechanisms by which this process is regulated against a multi-host pathogen remain unknown. Fusarium oxysporum is a model multi-host pathogen, known to be associated with neuronal stress in humans and vascular wilt in plants. The interaction between innate immune and neuronal pathways is the basis of many diverse biological responses. How these processes are coordinated in response to fungal disease is not well understood. Here, we show that F. oxysporum f. sp. ciceri causes neuronal stress and intestinal disintegration, ultimately leading to the death of Caenorhabditis elegans. To explore the regulatory framework of Fusarium-associated disease, we analysed the gene expression during infection, integrated temporal gene expression, and network analysis with genetic inactivation data in Caenorhabditis elegans. We identified 1024 genes showing significant changes in expression (corrected P-values <0.05) in response to Fusarium infection. Co-expression network analysis of our data identified prognostic genes related to disease progression. These genes were dynamically expressed in various neuronal and non-neuronal tissues exhibiting diverse biological functions, including cellular homeostasis, organ patterning, stress response, and lipid metabolism. The RNA-seq analysis further identified shared and unique signalling pathways regulated by DAF-16/FOXO and SIR-2.1 linking neuronal stress, which facilitates negative regulation of intestinal innate immunity. Genetic analysis revealed that GCY-5 in ASE functions upstream of DAF-16, whereas ASI-specific SRD-1 regulates behavioural immunity. Overall, our results indicate that a ubiquitous response occurs during Fusarium infection mediated by highly conserved regulatory components and pathways, which can be exploited further for the identification of disease-responsive genes conserved among animals and plants. Finally, this study provided a novel insight into cross-species immune signalling and may facilitate the discovery of cellular therapeutic targets for Fusarium-associated disease.en_US
dc.description.sponsorshipThis work was supported by grants from the Department of Biotechnology (DBT) (BT/HRD/35/01/05/2013), Government of India and the National Institute of Plant Genome Research, New Delhi, India to SC. PA is the recipient of pre-doctoral fellowship from the Council of Scientific and Industrial Research (CSIR), Govt. of India. KN is the recipient of postdoctoral fellowship from the Department of Biotechnology (DBT), Govt. of India. We thank the Caenorhabditis Genetics Centre (Univ. of Minnesota) for strains used in this study. We also thank Mr Jasbeer Singh for illustrations and graphical representations in the manuscript.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.subjectFungal infectionen_US
dc.subjectTranscriptomicsen_US
dc.subjectFusariumen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectneuronal and non-neuronal genesen_US
dc.subjectDAF-16-mediated immuneen_US
dc.titleInterplay of neuronal and non-neuronal genes regulates intestinal DAF-16-mediated immune response during Fusarium infection of Caenorhabditis elegansen_US
dc.typeArticleen_US
dc.identifier.officialurlhttps://www.nature.com/articles/cddiscovery201773en_US
dc.identifier.doi10.1038/cddiscovery.2017.73en_US
Appears in Collections:Institutional Publications

Files in This Item:
File Description SizeFormat 
Chakraborty S_2017_5.pdf
  Restricted Access
4.63 MBAdobe PDFView/Open Request a copy


Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.