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Title: | KIX domain of AtMed15a, a Mediator subunit of Arabidopsis, is required for its interaction with different proteins |
Authors: | Kumar, Vinay Waseem, Mohd Dwivedi, Nidhi Maji, Sourobh Kumar, Angad Thakur, Jitendra K. |
Keywords: | Mediator Med15 KIX domain transcription factors transactivation |
Issue Date: | 2018 |
Publisher: | Taylor & Francis Group |
Citation: | Plant Signaling & Behavior, 13(2): e1428514 |
Abstract: | Med15 is an important subunit of Mediator tail module and is characterized by a KIX domain present in the amino terminal. In yeast and metazoans, Med15 KIX domain has been found to interact with various transcription factors, regulating several processes including carbohydrate metabolism, lipogenesis, stress response and multidrug resistance. Mechanism of Med15 functioning in Arabidopsis is largely unknown. In this study, interactome of Arabidopsis Med15, AtMed15a, was characterized. We found 45 proteins that interact with AtMed15a KIX domain, including 11 transcription factors, 3 single strand nucleic acid-binding proteins and 1 splicing factor. The third helix of the KIX domain was found to be involved in most of the interactions. Mapping of the regions participating in the interactions revealed that the activation domain of a transcription factor, UKTF1 interacted with AtMed15a KIX domain. Thus, our results suggest that in Arabidopsis, activation domain of transcription factors target KIX domain of AtMed15a for their transcriptional responses. |
Description: | Accepted date: 26 December 2017 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/825 |
ISSN: | 1559-2324 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Thakur JK_2018_1.pdf Restricted Access | 1.41 MB | Adobe PDF | View/Open Request a copy |
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