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DC Field | Value | Language |
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dc.contributor.author | Kryvoruchko, Igor S. | - |
dc.contributor.author | Routray, Pratyush | - |
dc.contributor.author | Sinharoy, Senjuti | - |
dc.contributor.author | Torres-Jerez, Ivone | - |
dc.contributor.author | Tejada-Jiménez, Manuel | - |
dc.contributor.author | Finney, Lydia A. | - |
dc.contributor.author | Nakashima, Jin | - |
dc.contributor.author | Pislariu, Catalina I. | - |
dc.contributor.author | Benedito, Vagner A. | - |
dc.contributor.author | González-Guerrero, Manuel | - |
dc.contributor.author | Roberts, Daniel M. | - |
dc.contributor.author | Udvardi, Michael K. | - |
dc.date.accessioned | 2018-04-03T07:18:38Z | - |
dc.date.available | 2018-04-03T07:18:38Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Plant Physiology, 176(3): 2315-2329 | en_US |
dc.identifier.issn | 1532-2548 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/845 | - |
dc.description | Accepted date: December 21, 2017 | en_US |
dc.description.abstract | Iron (Fe) is an essential micronutrient for symbiotic nitrogen fixation in legume nodules, where it is required for the activity of bacterial nitrogenase, plant leghemoglobin, respiratory oxidases, and other Fe proteins in both organisms. Fe solubility and transport within and between plant tissues is facilitated by organic chelators, such as nicotianamine and citrate. We have characterized a nodule-specific citrate transporter of the multidrug and toxic compound extrusion family, MtMATE67 of Medicago truncatula. The MtMATE67 gene was induced early during nodule development and expressed primarily in the invasion zone of mature nodules. The MtMATE67 protein was localized to the plasma membrane of nodule cells and also the symbiosome membrane surrounding bacteroids in infected cells. In oocytes, MtMATE67 transported citrate out of cells in an Fe-activated manner. Loss of MtMATE67 gene function resulted in accumulation of Fe in the apoplasm of nodule cells and a substantial decrease in symbiotic nitrogen fixation and plant growth. Taken together, the results point to a primary role of MtMATE67 in citrate efflux from nodule cells in response to an Fe signal. This efflux is necessary to ensure Fe(III) solubility and mobility in the apoplasm and uptake into nodule cells. Likewise, MtMATE67-mediated citrate transport into the symbiosome space would increase the solubility and availability of Fe(III) for rhizobial bacteroids. | en_US |
dc.description.sponsorship | We thank Xiaofei Cheng and JiangQi Wen for their assistance with isolation of Tnt1 mutants; Shulan Zhang for help with experiments; Frank Coker, Colleen Elles, Janie Gallaway, and Vicki Barrett for greenhouse support with M. truncatula Tnt1 lines; and Mark Taylor for backcrossing the mutants. Pascal Ratet, Kirankumar S. Mysore, and Million Tadege are acknowledged for construction of the Tnt1 mutant resource. We also thank Lina Yang for help with preparation of a high-resolution image for Figure 1. We appreciate the support from Jeremy Murray and Christopher Town with acquisition of genomic sequences. We also thank Jeremy Murray, Maria Harrison, Rebecca Dickstein, and Carroll Vance for data exchange and critical feedback. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Society of Plant Biologists | en_US |
dc.subject | Iron-Activated Citrate Transporter | en_US |
dc.subject | Symbiotic Nitrogen Fixation | en_US |
dc.subject | MtMATE67 | en_US |
dc.title | An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | http://www.plantphysiol.org/content/176/3/2315 | en_US |
dc.identifier.doi | https://doi.org/10.1104/pp.17.01538 | en_US |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Sinharoy S_2018_1.pdf Restricted Access | 2.5 MB | Adobe PDF | View/Open Request a copy |
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