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DC Field | Value | Language |
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dc.contributor.author | Ghorai, Priyanka | - |
dc.contributor.author | Irfan, Mohammad | - |
dc.contributor.author | Narula, Alka | - |
dc.contributor.author | Datta, Asis | - |
dc.date.accessioned | 2018-08-24T06:41:49Z | - |
dc.date.available | 2018-08-24T06:41:49Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Applied Microbiology and Biotechnology, 102(22): 9731-9743 | en_US |
dc.identifier.issn | 1432-0614 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/882 | - |
dc.description | Accepted date: 2 August 2018 | en_US |
dc.description.abstract | The morphological plasticity of Candida albicans is a virulence determinant as the hyphal form has significant roles in the infection process. Recently, phosphoregulation of proteins through phosphorylation and dephosphorylation events has gained importance in studying the regulation of pathogenicity at the molecular level. To understand the importance of phosphorylation in hyphal morphogenesis, global analysis of the phosphoproteome was performed after hyphal induction with elevated temperature, serum, and N-acetyl-glucosamine (GlcNAc) treatments. The study identified 60, 20, and 53 phosphoproteins unique to elevated temperature-, serum-, and GlcNAc-treated conditions, respectively. Distribution of unique phosphorylation sites sorted by the modified amino acids revealed that predominant phosphorylation occurs in serine, followed by threonine and tyrosine residues in all the datasets. However, the frequency distribution of phosphorylation sites in the proteins varied with treatment conditions. Further, interaction network-based functional annotation of protein kinases of C. albicans as well as identified phosphoproteins was performed, which demonstrated the interaction of kinases with phosphoproteins during filamentous growth. Altogether, the present findings will serve as a base for further functional studies in the aspects of protein kinase-target protein interaction in effectuating phosphorylation of target proteins, and delineating the downstream signaling networks linked to virulence characteristics of C. albicans. | en_US |
dc.description.sponsorship | This work is financially supported by grants from Council of Scientific and Industrial Research, Department of Biotechnology and the Core Grant of National Institute of Plant Genome Research, New Delhi, India. P.G. and M.I. acknowledge Council of Scientific and Industrial Research, India, for the Senior Research Fellowship and Senior Research Associateship, respectively. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature | en_US |
dc.subject | Candida albicans | en_US |
dc.subject | Hyphal development | en_US |
dc.subject | Phosphoproteome | en_US |
dc.subject | TiO2 | en_US |
dc.subject | LC–MS/MS | en_US |
dc.subject | Protein kinase | en_US |
dc.title | A comprehensive analysis of Candida albicans phosphoproteome reveals dynamic changes in phosphoprotein abundance during hyphal morphogenesis | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://link.springer.com/article/10.1007%2Fs00253-018-9303-z | en_US |
dc.identifier.doi | https://doi.org/10.1007/s00253-018-9303-z | en_US |
Appears in Collections: | Institutional Publications |
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Datta A_2018_1.pdf Restricted Access | 2.27 MB | Adobe PDF | View/Open Request a copy |
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