Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/940
Title: | Improved method of RNA isolation from laser capture microdissection (LCM)-derived plant tissues |
Authors: | Gautam, Vibhav Singh, Archita Singh, Sharmila Verma, Swati Sarkar, Ananda K. |
Keywords: | LCM Laser microdissection RNA isolation Gene expression miRNA Root meristem |
Issue Date: | 2019 |
Publisher: | Springer Nature |
Citation: | Methods in Molecular Biology, 1933: 89-98 |
Abstract: | Laser capture microdissection (LCM) is a tool to isolate desired and/or less accessible cells or tissues from a heterogeneous population. In the current method, we describe an efficient and cost-effective method to obtain both high-quality mRNA and miRNAs in sufficient quantity from LCM-derived plant tissues. The quality of the isolated RNA can be assessed using Bioanalyzer. Using modified stem-loop RT-PCR, we confirmed the presence of 21–24 nucleotide (nt) long mature miRNAs. This modified LCM-based method has been found to be suitable for the tissue-specific expression analysis of both genes and small RNAs (miRNAs). |
Description: | Accepted date: 04 April 2019 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/940 |
ISBN: | 978-1-4939-9045-0 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sarkar AK_2019_2.pdf Restricted Access | 261.45 kB | Adobe PDF | View/Open Request a copy |
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