Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/944
Title: | Improved laser capture microdissection (LCM)‑based method for isolation of RNA, including miRNA and expression analysis in woody apple bud meristem |
Authors: | Verma, Swati Gautam, Vibhav Sarkar, Ananda K. |
Keywords: | Bud meristem Gene expression Laser capture microdissection Malus MicroRNAs RNA isolation Small RNA Stem-loop RT-PCR Tree |
Issue Date: | 2019 |
Publisher: | Springer Nature |
Citation: | Planta, 249(6): 2015-2020 |
Abstract: | Isolation of high-quality RNA, including miRNA, from microscopic woody apple bud meristem using laser capture microdissection-based method. It is often challenging to study the expression of microRNAs (miRNAs) or genes in less accessible inner tissues of tree species rich in polyphenols or polysaccharides. Here, we report a laser capture microdissection (LCM)-based method for efficient and cost-effective isolation and expression analysis of miRNAs and genes in the meristem tissue of woody apple bud. The tissue fixation, processing, infiltration, and sectioning steps were optimized for LCM-based excision and subsequent RNA isolation. Further, we have confirmed that RNA isolated from LCM-derived apple bud meristem contained miRNAs and was of good quantity and quality, sufficient for downstream expression analysis. |
Description: | Accepted date: 4 March 2019 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/944 |
ISSN: | 1432-2048 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sarkar AK_2019_4.pdf Restricted Access | 1.16 MB | Adobe PDF | View/Open Request a copy |
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