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DC Field | Value | Language |
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dc.contributor.author | Meena, Mukesh Kumar | - |
dc.contributor.author | Prajapati, Ramgopal | - |
dc.contributor.author | Krishna, Deepthi | - |
dc.contributor.author | Divakaran, Keerthi | - |
dc.contributor.author | Pandey, Yogesh | - |
dc.contributor.author | Reichelt, Michael | - |
dc.contributor.author | Mathew, M.K. | - |
dc.contributor.author | Boland, Wilhelm | - |
dc.contributor.author | Mithöfer, Axel | - |
dc.contributor.author | Vadassery, Jyothilakshmi | - |
dc.date.accessioned | 2019-05-13T09:50:18Z | - |
dc.date.available | 2019-05-13T09:50:18Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Plant Cell, 31(7): 1539-1562 | en_US |
dc.identifier.issn | 1532-298X | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/948 | - |
dc.description | Accepted date: May 7, 2019 | en_US |
dc.description.abstract | Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis thaliana activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intra-vascular Ca2+ fluxes. CNGC19 is a Ca2+ permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cAMP and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defence in cngc19 mutants leads to a decrease in herbivory-induced JA-Ile biosynthesis and expression of JA responsive genes. cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4 (BCAT4), which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 (CaM2) in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling. | en_US |
dc.description.sponsorship | This work was funded by the Department of Biotechnology (DBT), India through NIPGR core and BioCare grants (BT/PR18059/BIC/101/994/2016), MPG-India partner group program of the Max Planck Society (Germany) and Department of Science and Technology (DST), India (DST/INT/MPG/P-28/2015). We acknowledge DBT-JRF for funding R.P, National Bureau of Agricultural Insect Resources (NBAIR), Bangalore for S. litura eggs (National Accession No. is: NBAII-MP-NOC-02), Pradeep Kumar Maurya (NIPGR) for rearing Spodoptera, NIPGR central instrumentation and phytotron facility, DBT-eLibrary Consortium (DeLCON) for providing access to e-resources, Sahil Lall and Central Imaging and Flow Facility (NCBS) for Xenopus oocyte images, Dr. Suneel Katariya (JNU) for help with cAMP quantification, Monika Heyer and Andrea Lehr (MPI-CE) for help with phytohormone extraction, Prof. Dale Sanders (JIC, UK) for GCaMP3 lines, Prof. Gerald Berkowitz (University of Connecticut, USA) for pepr1 pepr2 mutant, Prof. Stefan Binder (University of Ulm, Germany) for bcat4 mutant line SALK_013627 and Dr. Guillermo Hugo Jimenez-Aleman (MPI-CE, Jena) for chemically synthesized JA-Ile. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Society of Plant Biologists | en_US |
dc.subject | Ca2+ | en_US |
dc.subject | Spodoptera Herbivory | en_US |
dc.subject | CNGC19 | en_US |
dc.subject | Arabidopsis | en_US |
dc.title | The Ca2+ channel CNGC19 regulates Arabidopsis defense against spodoptera herbivory | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | http://www.plantcell.org/content/early/2019/05/10/tpc.19.00057 | en_US |
dc.identifier.doi | https://doi.org/10.1105/tpc.19.00057 | en_US |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Vadassery J_2019_1.pdf Restricted Access | 39.88 MB | Adobe PDF | View/Open Request a copy |
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