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http://223.31.159.10:8080/jspui/handle/123456789/948
Title: | The Ca2+ channel CNGC19 regulates Arabidopsis defense against spodoptera herbivory |
Authors: | Meena, Mukesh Kumar Prajapati, Ramgopal Krishna, Deepthi Divakaran, Keerthi Pandey, Yogesh Reichelt, Michael Mathew, M.K. Boland, Wilhelm Mithöfer, Axel Vadassery, Jyothilakshmi |
Keywords: | Ca2+ Spodoptera Herbivory CNGC19 Arabidopsis |
Issue Date: | 2019 |
Publisher: | American Society of Plant Biologists |
Citation: | Plant Cell, 31(7): 1539-1562 |
Abstract: | Cellular calcium elevation is an important signal used by plants for recognition and signaling of environmental stress. Perception of the generalist insect, Spodoptera litura, by Arabidopsis thaliana activates cytosolic Ca2+ elevation, which triggers downstream defense. However, not all the Ca2+ channels generating the signal have been identified, nor are their modes of action known. We report on a rapidly activated, leaf vasculature- and plasma membrane-localized, CYCLIC NUCLEOTIDE GATED CHANNEL19 (CNGC19), which activates herbivory-induced Ca2+ flux and plant defense. Loss of CNGC19 function results in decreased herbivory defense. The cngc19 mutant shows aberrant and attenuated intra-vascular Ca2+ fluxes. CNGC19 is a Ca2+ permeable channel, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cAMP and Ca2+ results in Ca2+ influx. Breakdown of Ca2+-based defence in cngc19 mutants leads to a decrease in herbivory-induced JA-Ile biosynthesis and expression of JA responsive genes. cngc19 mutants are deficient in aliphatic glucosinolate accumulation and hyperaccumulate its precursor, methionine. CNGC19 modulates aliphatic glucosinolate biosynthesis in tandem with BRANCHED-CHAIN AMINO ACID TRANSAMINASE4 (BCAT4), which is involved in the chain elongation pathway of Met-derived glucosinolates. Furthermore, CNGC19 interacts with herbivory-induced CALMODULIN2 (CaM2) in planta. Together, our work reveals a key mechanistic role for the Ca2+ channel CNGC19 in the recognition of herbivory and the activation of defense signaling. |
Description: | Accepted date: May 7, 2019 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/948 |
ISSN: | 1532-298X |
Appears in Collections: | Institutional Publications |
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Vadassery J_2019_1.pdf Restricted Access | 39.88 MB | Adobe PDF | View/Open Request a copy |
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